Pune University BE Chemical Engineering Bioprocess Engineering
B.E. (Chemical Engineering) BIOPROCESS ENGINEERING (2008 Pattern)
(Elective – I) (Sem. – I)
Time: 3 Hours] [Max. Marks :100
Instructions to the candidates:
1) Answer 3 questions from Section -1 and 3 questions from Section – II.
2) Answers to the two sections should be written in separate books.
3) Neat diagrams must be drawn wherever necessary.
4) Figures to the right indicate full marks.
5) Use of electronic pocket calculator is allowed.
6) Assume suitable data, if necessary.
SECTION – I
Q1) a) Explain specific growth rate of bacteria. 
b) Discuss the concept of Biorefinary. 
Q2) Write short notes on following : 
a) Amino acid building blocks and polypeptides.
b) Proteins and their diverse biological applications.
Q3) Explain the manufacturing process for 
a) Acetic acid and
Q4) Explain the manufacturing process for 
a) Acetone and
b) Lactic Acid.
Q5) a) Explain the significance of the constants in the Michaelis Menten equation and describe different ways of determining them. 
b) Discuss the different cases of enzyme reactions in heterogeneous systems.
Q6) a) Explain the mechanisms of catalysis of enzyme acting on two substrates. How are the kinetics determined in this case? 
b) The following experimental data were collected during a study of the catalytic activity of an intestinal peptidase with the substrate glycylglycine. 
|Glycylglycine + H2O ——- > 2 glycine
Use graphical analysis to determine the V and K for this enzyme
or’ j max m «/
preparation and substrate.
SECTION – II
Q7) a) What is dilution rate? Discuss the effect of dilution rate on biomass and substrate concentrations? 
b) Write a note on simple product formation kinetics. 
Q8) a) Derive design equation for CSTR for continuous cultivation of cells and also explain Monod kinetics. 
b) What are other environmental effects on growth of microbial biomass? Discuss each factor in detail. 
Q9) a) Explain the fed batch mode of fermenter operation in terms of features, advantages, disadvantages, concentration time profiles, mass balances and examples. 
b) What is meant by critical oxygen concentration and its significance with respect to cell growth? 
Q10)a) What is immobilization of enzymes? What are its advantages over free enzymes? 
b) Discuss and compare mechanically agitated contactor and bubble column reactor as fermenter. 
Q11)a) Describe the steps involved in the modeling and simulation of bioprocesses. 
b) Discuss solvent extraction with examples used in bioseparations. 
Q12)Write short notes on : 
a) Bioprocess economics.
b) Fluidized bed bioreactor.
c) Crystallization and drying.
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