Pune University BE Exam Paper
B.E. (Biotechnology) INTRODUCTION TO SYSTEMS BIOLOGY
(2008 Pattern) (Sem. – II)
Time :3 Hours] [Max. Marks :100
Instructions to the candidates:
1) Answer three questions from section I and three questions from section II.
2) Answers to the two sections should be written in separate answer books.
3) Draw neat diagrams wherever necessary.
4) Pigures to the right indicate full marks.
SECTION – I
Q1 What is Human Genome Project? Enlist the different phases involved in it. In what ways did the Human Genome project impact biology? 
Q2) What is the Central dogma of Molecular biology? Mention the different components of systems biology and describe the four distinct phases which lead to system level understanding. 
Q3) How and in which fields has genomics transformed the outlook. 
Q4) Describe in detail pyrosequencing technique? What is the approach taken by the Roche 454 sequencer, give a detailed summary. Write a note on Genome annotation. 
Q5) What is a microarray? Give the various types of microarrays available in the market. What are the applications of microarrays? 
Q6) Write a short note on RNAi technology and its real world applications. 
SECTION – II
Q7) Write a short note on epigenetics. What are the different types of modifications observed which lead to epigenetic inheritance of various traits? 
Q8) Comment on Epigenetics and disease with respect to prions causing disease in mammals. 
Q9) Describe pharmacogenomics as an upcoming field. What are the different benefits and the barriers to drugs developed using the pharmacogenomic approach. 
QI 0) Write a note on methods for drug delivery, drug metabolism and excretion, what are slow metabolisers and extensive metabolisers. 
QII) Describe in detail any two of the techniques (8 marks each) : 
a) 2-D Gel electrophoresis.
b) MALDI-TOF analyzers.
c) Mass spectrometry.
QI2) What is a metabolome. What are the different techniques used in metabolomics study, comment on the use and applications of metabolomics.