Pune University BE (Biotechnology Engineering) Bio Therapeutics Technology Question Papers
B.E. (Biotechanology) BIO-THERAPEUTICS TECHNOLOGY (2008 Course) (415461)
Time :3 Hours] [Max. Marks :100
Instructions to the candidates :-
1) Answer 3 questions from each Section I and 3 question from Section II.
2) Answers to the two sections should be written in separate books.
3) Neat diagrams must be drawn wherever necessary.
4) Pigures to the right indicate full marks.
5) Make necessary assumptions wherever necessary.
6) Use of electronic pocket calculator is allowed.
SECTION – I
QI) Define following: 
i) Factor of Safety ii) Young’s Modulus
i) Stress Concentration iv) Fatigue
i) Creep vi) Endurance Limit
i) Poisson’s Ratio viii) Polar Moment of Inertia
Q2) What are the various types of theories of elastic failure? Explain each. 
Q3) a) Write a note on general design consideration. 
b) Explain multi-shell construction of high pressure vessel. 
c) Write a short note on purging of vessels. 
Q4) a) Explain auto frottage and shrink fit construction for high pressure vessels. 
b) A vessel is to be designed to withstand an internal pressure of 150 MN/m2. An internal diameter of 30 cm is specified, and steel having yield point of 450 MN/m2 has been selected. Calculate the wall thickness required by the various theories with a factor of safety 1.5 and ^ = 0.3. 
Q5) a) Explain different types ofjackets used in construction of reaction vessel.
b) Explain shaft design based on Torque and bending moment. 
Q6) a) Explain any four types of agitators with neat sketch. b) Write a short note on jackets and coils with a neat sketch. 
SECTION – II
Q7) a) A single effect evaporator is to be operated at absolute pressure of 0.13 bar. Estimate the heat transfer area necessary to concentrate 4500 kg/hr of caustic soda solution from 10% to 40% (by weight) using saturated steam at 1170C as heating media. The overall heat transfer coefficient may be taken as 1.25 kW/m^C. 
Data : Specific heat of feed = 4000 J/Kg0C
Specific heat of Product = 3260 J/Kg0C
Feed temperature = 180C
BPR of Solution = 300C
Density of Boiling liquid = 1390 kg/m3
The liquid level in the evaporator is 1200 mm above the heating surface.
b) Explain various methods of feeding for multiple effect evaporators. 
Q8) a) What are the advantages of plate heat exchanger? With neat sketches show the various flow patterns in plate heat exchanger. 
b) Explain design of Long tube vertical evaporators with neat sketch. 
Q9) a) Explain the design of liquid distributors and redistributors. 
b) Discuss various feed arrangements to be considered for distillation column along with neat sketches. 
QIO) a) Explain segmental downcomer, chord type downcomer, circular type downcomer with neat sketches. 
b) Explain design variables in distillation column. 
QII)What are various types of filter used in biotechnological industry? Explain any four. 
Q12AWrite short notes on : 
a) Steam sterilization procedures
b) F ilter integrity testing
c) Validation of filters
d) Tangential flow filtration.