Pune University BE Biotechnology Bioseparation-II Question Papers

Pune University Previous Papers

B.E. (Biotechnology) BIOSEPARATION – II

(2008 Pattern) (Sem. – I)

Time :3 Hours]                                                                                              [Max. Marks :100

Instructions to the candidates:

1)            Answer three questions from Section I and three questions from Section II.

2)            Answers to the two sections should be written in separate answer books.

3)          Neat diagrams must be drawn wherever necessary.

4)          Pigures to the right indicate full marks.

5)          Assume suitable data, if necessary.


QI) a) Explain in detail role and importance of downstream processing in biotechnological processes.                                                        [10]

b) What do you mean by purification? Describe problems and requirement of bio product purification.                                                                                                [6]


Q2) Write in detail about process design criteria for following classes of bio­products.    [16]

a)        High volume and Low value Products.

b)        Low volume and High value Products.

Q3) Write short notes on : (Any 2)                                                                       [16]

a)         Spectroflurometry.

b)        Spectrophotometry.

c)        Atomic absorption spectroscopy.

d)         NMR.


Q4) Explain in detail gel permeation chromatography with the help of following points :           [18]

a)        Principle.

b)        Retention.

c)        Procedure.

d)        Material.

e)        Application.


Q5) Describe in detail reversed phase chromatography and hydrophobic interaction chromatography.                                                                                                      [18]


Q6) Write short notes on : (Any 2)                                                                                  [16]

a)         Gas chromatography.

b)        Liquid chromatography.

c)         GC-MS.

d)        LC-MS.

Q7) What are hyphenated techniques? Illustrate the concept with examples. [16]


Q8) What do you mean by SFE? Explain in detail technique with case study. [16] Q9) a) Explain production of penicillin with respect to bioseparation techniques.


b)        Write a historical background of beer production and elaborate on advancement of the production process.                                                                                            [9]


QIO) Describe the process of butanol production and methods of butanol separation.     [18]

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