UPTU Previous Question Papers B Tech 6th Semester Genetic Engineering 2006-07

UPTU Previous Question Papers

B Tech 6th Semester Genetic Engineering 2006-07

 

Note : (1) Attempt all questions.

(2) All questions carry equal marks.

1.  Attempt any two parts of the following :

(a) What is difference between pBR322 and PUC19 vectors?

(b) What are the features of a good vector? Briefly describe the features of one plasmid and one phage vector of E. coli.

(c) Write short notes on the following : (any two)

(i) Plasmids,

(ii) Artificial chromosome and

(iii) Cosmids.

 

2.  Attempt any two parts of the following :

(a)     Give an illustrated account of gene cloning.

(b) What is genomic library? Briefly describe the procedure for preparing a cDNA library.

(c) PCR is a revolutionary technique that promises to replace gene cloning for many of the objectives. Comment with the help of suitable examples.

 

3.  Attempt any two parts of the following :

(a) Give a detailed account of Restriction endonucleases.

(b) What are the DNA enzymes? Discuss in detail their mode of action.

(c) Write short notes on the following : (any two)

(i) Terminal transferase

(ii) Alkaline phosphatase and

(iii) Palindromes.

 

4.  Attempt any two parts of the following :

(a) Briefly describe the various strategies used for the selection of cells carrying the recombinant vector after their transformation with a mixture of unchanged and recombinant vectors.

(b) Give a detailed account of gene cloning in eukaryotes.

(c) Give a detailed account of recombinants screening.

 

5.  Attempt any two parts of the following :

(a) What is DNA sequencing? Give a detailed account of Maxam-Gilbert method of DNA sequencing.

(b) Discuss in detail about different blotting techniques used for separation of DNA, RNA and proteins.

(c) Write short notes on following (any two) :

(i) Northern Blotting

(ii) Antisense gene technology and

(iii) Automated DNA sequencers.

 

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