UPTU Previous Question Papers
B Tech 4th Semester Enzymology 2006-07
Note : Attempt all five questions.
1. Attempt any four parts of following :
(a) What are enzymes? Why are they called biocatalyst?
(b) Differentiate between coenzymes and cofactors with example.
(c) What are multienzyme complexes? Explain with suitable examples.
(d) What are active sites? Explain.
(e) What are isoenzymes? Explain with example.
2. Attempt any two parts of the following :
(a) What do you understand by optimum pH and temperature of an enzyme? Explain.
(b) Define km and Vmaxl How can they be measured?
(c) Discuss briefly allosteric enzymes?
3. Attempt any one part of the following:
(a) What are industrial enzymes? Give commercial application of any two enzymes with their source.
(b) Differentiate between topoisomerase glucoisomerase. What is the function of lysozyme and its mechanism of action?
4. Attempt any one part of the following:
(a) What is enzyme immobilization? How is it carried out? What can be its application?
(b) Explain the molecular basis of structural stability of enzymes with respect to folding and defolding.
5. Attempt any one of the following:
(a) What are the techniques involved in isolation and purification of enzymes?
(b) Discuss in detail therapeutic application of enzymes.