UPTU Previous Exam Papers
B Tech 4th Semester Biochemistry 2006-07
Note : Attempt all questions.
All questions carry equal marks.
1 . Attempt any four of the following:
(a) What is the mirror image of a -D glucose?
(b) What does the a -prefix before an amino acid signify?
(c) Write down contribution of the following scientists: E.Fischer and Louis Pasteur.
(d) What is the basis of separation of molecules in gel permeation chromatography?
(e) Why hydrophobic interaction chromatography is generally carried out in the presence of high salt concentrations?
(f) Describe the various conditions required for enzyme activity.
2. Attempt any four of the following :
(a) Why glyconeogenesis is not a reversal of glycolysis?
(b) What are isozymes? Explain with special reference to LDH.
(c) How many moles of ATP formed during glycolysis if arsenate an analog of phosphate is present in the system? Explain the reaction involved in the process.
(d) How does the flow of glucose6-phosphate depends on the need for NADPH, ribose-5-phosphate and ATP?
(e) How does the oxidation and phosphorylation are coupled by ETC?
(f) Give a brief account of energy rich compounds and explain why are they called high energy compounds?
3. Attempt any two of the following :
(a) How does the odd chain fatty acids yield propionyl coenzyme A in the final thiolysis step?
(b) How does the microorganism use ATP and powerful reductant to convert N2 to NH4+ ?
(c) What are allosteric enzymes ? Explain with reference to ATCase ? How does final product of the pyrimidine pathway is inhibited by ATCase ?
4. Attempt any two of the following :
(a) Why bacteria and plants have an ability to grow on acetate or other compounds that yield acetylCoA?
(b) Write down routes for the metabolism of benzoate by different bacteria.
(c) Write down pathway of electron flow from H20 to NADP+ in photo-synthesis.
5. Attempt any two of the following :
(a) Compare oxygen binding curve of hemoglobin and myoglobin.
(b) How does the calcium sensors function?
(c) How does the toxic derivatives of 02 such as superoxide radical are scavenged by protective enzymes ?