# NIT Raipur Syllabus 3rd Semester Biotechnology Engineering

# NIT-RAIPUR

## III SEM BIOTECHNOLOGY SYLLABUS

Name of the Subject Biochemistry Subject Code BT20312BT

Semester 3rd Board of Studies Biotechnology

Maximum Marks 70 Minimum Marks 25

Lecture Periods/Week

Tutorial Periods/Week

Practical Periods/Week

Credits 03 01 03 4+2

Unit I- Biomolecules

Classification of Biomolecules. Structure and function of Biomolecules; Carbohydrates, Lipids, Nucleic

acids, and Protein.

Unit II –Biochemistry of Acid, Base, Water & Enzymes

Biochemistry of water, acid and base, Importance of buffer in homeostasis, Henderson-Hasselbach

equation; Enzymes: – Concepts of ligands, activation energy, action mechanism, inhibition, Michaelis-

Menton equation and its modification.

Unit III- Carbohydrate Metabolism

Glycolysis, Embden Meyerhoff pathway, TCA cycle, Gluconeogenesis, Glycogenesis, Glycogenolysis,

Pentose Phosphate Pathway, Electron transport chain.

Unit IV- Protein and Nucleic Acid metabolism

Urea cycle, Plasma protein and their clinical significance, Biosynthesis and catabolism of some vital

amino acid .Nucleic acid metabolism; Purine and Pyrimidine synthesis.

Unit V- Lipid Metabolism

Fatty acid biosynthesis, ?

– oxidation of fatty acid, Cholesterol metabolism, Steroid hormones and their

role.

Text books:

1. A.L. Lehninger, D.L.Nelson and M.M Cox- Principles of Biochemistry, Werth publishers 2000.

Reference books

1. L. Stryer, J.M. Berge, J.L.Tymozoeko- Biochemistry, W.H. Freeman and Co., 2002.

2. D. Voet and G. Voet- Biochemistry, John Wiley and sons 1994.

National Institute of Technology, Raipur (C.G.)

DEPARTMENT OF BIOTECHNOLOGY SYLLABUS

Name of the

Subject

Biophysics Subject Code BT20315BT

Semester 3

rd Board of Studies Biotechnology

Maximum Marks 70 Minimum Marks 25

Lecture

Periods/Week

Tutorial

Periods/Week

Practical

Periods/Week

Credits

03 01 00 4

Unit I – Introduction to Biophysics

; Concept of Biophysics, Division of Biophysics.

Introduction to Thermodynamics; First and Second law of Thermodynamics, Concept of

energy in Biological system in the light of Thermodynamics, Living body as a

thermodynamics system. Hydrogen ion concentration- Biological importance of Ph,

Experimental determination of Ph.

Unit II

– **Biophysical Principles: **Diffusion ; Kinetics of Diffusion, Fick’s law, Types of

Diffusion, Active transport, Osmosis, Osmoregulation, Membrane hydrolysis,

Endocytosis, Absorption, Adsorption, Viscosity, Surface Tension, Ultrafiltration.

Biological importance of acids, bases and salts .Henderson- hasselbalch equation.

Unit III – Hemodynamics:

General concept of viscosity for Newtonian and Non-

Newtonian fluid. Rheological behavior of blood. Differences between Newtonian and

Non- Newtonian fluid. Poiseuille’s law, Blood flow- Laminar flow, Turbulent flow.

Velocity of blood flow- Bernoulli’s principle. Methods of measurement of blood flow.

Unit IV- Physical techniques in structure determination

– X ray diffraction, Nuclear

Magnetic Resonance, Electron microscopy, Optical Microscopy, Compound

Microscopy, Mass Spectroscope, Infrared Spectroscope, UV Spectroscope, Lasers,

Chromatography, Electrophoresis.

Unit V- Biomechanics:

Cardiac mechanics; Heart as a pump, Mechanical properties of

blood vessels. Respiratory mechanics; Breathing mechanism. Structure and mechanical

properties of skin. Cardiac mechanics, Lubrication of joint. Bone mechanics.

National Institute of Technology, Raipur (C.G.)

Name of Text books:

1. Physical Biochemistry: David Friefelder, 5

th Ed, PHI

2. Practical Biochemistry principles and techniques: Editor Wilson and

Walker,Cambridge University Press.

3. Physical Biochemistry: Kensal E van Holde.PHI

Name of Reference books

1. A text book of Biophysics R.N.Roy, New Central Book Agency (P) Ltd.

2. Physical Biochemistry: Kensal E van Holde.PHI

National Institute of Technology, Raipur (C.G.)

DEPARTMENT OF BIOTECHNOLOGY SYLLABUS

Name of the Subject Cell & Molecular

Biology

Subject Code BT20313BT

Semester 3

rd Board of Studies Biotechnology

Maximum Marks 70 Minimum Marks 25

Lecture

Periods/Week

Tutorial

Periods/Week

Practical

Periods/Week

Credits

03 01 03 4+2

Unit I – Cell structure & function

Cell structure and function of organelles. Cell Cycle and its Regulation. Cell Division: Mitosis

and Meiosis.

Unit II – Membrane Transport

Transport phenomenon across cell membrane and its various types. Signal Transduction- cyclic

AMP, IP3, G-Protein, Ca2+ ions, Enzyme linked cell surface receptors.

Unit III -Genetics

Mendel’s Laws, Linkage and Crossing Over, DNA as genetic material, Structure of DNA and

RNA, Organization of DNA in chromosomes, Chromosome Aberrations, Special chromosomes,

Genes, DNA Replication.

Unit IV – Gene Expression & Mutation

Transcription, Translation of Prokaryotes and Eukaryotes, Genetic code, Gene Expression in

prokaryotes and eukaryotes- Regulation, Mutations: Types, Mutants

Unit V – Gene Transfer

Disorders due to chromosomal change and gene mutation, Transposons, Retrotransposons, Gene

transfer mechanisms in Transformation, Transduction and Conjugation.

Text Books:

1. Cell and Molecular Biology- Concepts and Experiments, Gerald Karp, John Wiley and Sons

2.Cell and Molecular Biology- S.C Rastogi.

Reference Books:

1. Genes IX, Benjamin Lewin

2. Voet and Voet, Biochemistry, John Wiley and Sons

3. Nelson and Cox- Lehninger, Principles of Biochemistry, Werth Publishers

4. Fundamentals of Genetics, Simmons and Snustad.

National Institute of Technology, Raipur (C.G.)

DEPARTMENT OF BIOTECHNOLOGY SYLLABUS

Name of the Subject Mathematics III Subject Code MA20311BT

Semester 3

rd Board of Studies Mathematics

Maximum Marks 70 Minimum Marks 25

Lecture

Periods/Week

Tutorial

Periods/Week

Practical

Periods/Week

Credits

03 01 00 04

Unit I – FOURIER SERIES AND FOURIER TRANSFORM

Expansion of function as Fourier series, Change of interval, Even and odd functions, Half-range Fourier

series, Practical harmonic analysis, Fourier Sine and Cosine transforms, Properties of Fourier transform,

Inverse Fourier transform, Fourier transform of derivatives.

Unit II – LAPLACE TRANSFORM

Laplace transform of elementary functions, properties of Laplace transform, Laplace transform of

derivatives and integrals, multiplication by tn and division by t, Laplace transform of periodic functions,

Inverse Laplace transform, Convolution theorem, Laplace transform of unit step function and Dirac

delta function, Application of Laplace transform to solutions of ordinary differential equations.

Unit III – THEORY OF COMPLEX VARIABLES

Limit, Derivative and Analytic functions; Cauchy- Riemann equations and its applications to flow

problems; complex Integration: Line and Contour integral, Cauchy integral theorem and Integral

formula; Taylor series, Laurent series, singularities; Poles and their orders ans residues; Evaluation of

real definite integrals.

Unit IV – PARTIAL DIFFRENTIAL EQUATION

Formation of partial differential equations, Lagrange’s solution of first order linear partial differentiation

equation, Homogeneous and Non-homogeneous linear partial differential: Non-linear partial differential

of first order, Charpit’s method and Jacobi’s method, Solution of partial differential equations by the

method of separation of variables.

Unit V – PROBABILITY AND SAMPLING DISTRIBUTIONS

Discrete and Continuous probability distributions, Chebyshev’s theorem, Binomial distribution , Poisson

distribution, Normal distribution, Gamma distribution and exponential distribution, Relation between

exponential, gamma and poisson distribution, Sampling distribution, sampling distribution of mean,

variances, differences and sums.

National Institute of Technology, Raipur (C.G.)

Text books

1. Higher engineering mathematics by Dr. B.S.Grewal- Khanna Publishers

2. Advanced Engg. Mathematics by Erwin Kreyszig- John Wiley and Hill Publishing

3. Higher Engg Mathematics by B V Ramana- Tata Mc Graw Hill Publishing

4. Elements of partial differential equations by I N Sneddan-Mc- Graw Hill International Editions

Reference Books:

1. Advanced engg. Mathematics by R.K. Jain and S R K Iyenger- Narosa Publishing house

2. Appied Mathematics by P.N. Wartikar and J.N Wartikar. Volume-II Pune Vidyarthi Griha Prakashan,

Pune.

3. Applied Mathematics for engineers and Physicists by Louis A. Pipes -TMH

National Institute of Technology, Raipur (C.G.)

National Institute of Technology, Raipur (C.G.)

DEPARTMENT OF BIOTECHNOLOGY SYLLABUS

Name of the

Subject

Object Oriented

Programming

Subject Code IT20314BT

Semester 3

rd Board of Studies Information

Technology

Maximum Marks 70 Minimum Marks 25

Lecture

Periods/Week

Tutorial

Periods/Week

Practical

Periods/Week

Credits

03 01 03 4+2

Unit I : Introduction

: Basic concepts of OOP, Benefits of OOP, Object Oriented

Language, Structure of C++ Program, Compiling & Linking , Operators & Expressions,

Looping concepts, Arrays & Structures, functions.

Unit II : Classes & Object

: Specifying a class, Define member function, Scope of class

and its member , Nested Class, Data hiding & encapsulation, Friend function, Array

within a class, array of object as function argument, function returning object, static

member.

Unit III: Constructors and Destructors

: Constructor function, parameterized multiple

constructor, default constructor, copy constructor, const and class, Data conversion

between objects of different classes, Destructor function, Polymorphism, function

overloading, Operator overloading.

Unit IV: Inheritance, Pointer & Virtual function

: Define derived classes, single

inheritance, multilevel inheritance, Hierarchical inheritance, Hybrid Inheritance, Pointers

to objects, this pointer, Pointers to derived class, Virtual function, Pure Virtual function,

Abstract classes.

Unit V: File I/O & Templates

: files streams, opening & closing a file, read () & write()

functions, detecting end-of-file, seekp(), seekg(), tellg(), tellp()function. Introduction to

Templates & Exception,

Creating and handling Templates and Exception in OOP.

Name of Text Books

1.

OOPS with C++ : E. Balagurusamy

2. OOP with C++: Robort Laphore.

Name of Reference Books

1. Object Oriented Programming in C++ : StroutStrups.

2. Programming with C++ : Venugopal .

3. Programming with C++ : D Ravichandran

4. Let us C++ : Yashwant Kanetkar.

National Institute of Technology, Raipur (C.G.)

5. C++ and OOPs Paradigm by Debasish Jana ( PHI)

6. OOP-P Sengupta & B.B. Choudhari ( PHI)

7. OOP with C++ by M.P. Bhave & S. A. Patekar ( Pearson Education)

8. OOP with C++ : Poonamchanda Sarang ( PHI)

National Institute of Technology, Raipur (C.G.)

DEPARTMENT OF BIOTECHNOLOGY SYLLABUS

Name of the

Subject

Stoichiometric

Calculations

Subject Code BT20316BT

Semester 3

rd Board of Studies Biotechnology

Maximum Marks 70 Minimum Marks 25

Lecture

Periods/Week

Tutorial

Periods/Week

Practical

Periods/Week

Credits

04 01 00 05

Unit I – Unit and its conversion

: Normality, Molality, Molarity, PVT behaviors, Gas

laws, Partial pressure and pure component volume methods. Limiting and excess

reactant.

Unit II – Humidity and Saturation:

Percentage Humidity, Relative Humidity, Molal

Humidity, Cox Chart. Crystallization.

Unit III – Combustion calculations:

fuel and flue gas analysis, air fuel ratio, theoretical

oxygen, percentage excess air, limiting and excess reactant.

Unit IV- Material Balance:

Material balance involving physical changes, chemical

changes and biochemical reactions, chemical reactions, Recycle, bypass and purge

operations.

Unit V – Energy Balance calculations:

Thermo physics, Heat capacity calculations,

Enthalpy changes of chemical and biochemical reactions, Dissolution and Laws of

Thermo chemistry. Effect of pressure and temperature on heat of reaction.

Name of Text Books:

1. Bhatt and Vora, ‘Stoichiometry’, McGraw Hill Publications

2. Hougen and Watson, “Chemical Process Principles Part – I Material and Energy

Balance 2

nd Edition

Name of Reference Books:

1. K A Gavhane, Unit operations, Nirali publication

2. Himmelblaue, basic principles and calculations in Chemical engineering, Prentice Hall

3. Ghoshal,Sanyal,Dutta, Introduction to chemical engineering]

Subject: Biochemistry Lab

Subject Code: BT20321BT

End Semester Marks : 20

List of Experiments:

1

**. **Identification of given sample (Carbohydrate /Protein).

2. Estimation of carbohydrate.

3. Separation of amino acid by paper chromatography.

4. Estimation of Protein.

5. SDS-PAGE of protein.

6. Determination of Iodine value of fat.

7. Determination of saponification value of fat.

8. Isolation of DNA from plant cells

9. Isolation of RNA from plant cells

10. Citric Acid production by fermentation.

11. Isolation of Amylase Producing organisms.

12. Catalase production test.

13. Voges –Proskauer test.

14. Qualitative test for amylase activity

15. Detection of proteolytic enzymes produced by microorganisms on milk agar plate

List of Equipments/Machine Required:

1. Colorimeter

2. Spectrophotometer

3. Water Bath

4. Digital Balance

5. Electrophoresis apparatus for protein, DNA and RNA separation

6. Incubator

Recommended Books:

1. Biochemical Methods by Sadasivam and Manickam

2. Laboratory Manual in Biochemistry by Jayaraman

3. Refer Books mentioned in theory syllabus

Subject: Cell And Molecular Biology Lab

Subject Code : BT20322BT

End Semester Marks : 20

List of Experiments:

1

**. **Study of different type of fungal and bacterial cell.

2. Study of unicellular organisms in pond water.

3. Study of leaf structure by section cutting.

4. Study of stem structure by section cutting.

5. Study of cell division in root tip/onion bud.

6. Isolation of DNA from onion Cell.

7. Measurement of cell size by ocular Microbiology.

8. Hanging drop method for studying motility of microorganisms from sewage water.

9. Determination of Leukocyte Cell.

10. R.B.C. Counting.

11. W.B.C. Counting.

List of Equipments/Machine Required:

1. Microscope

2. Micrometer (Ocular and stage)

3. Haemocytometer

4. Ultra-centrifuge

Recommended Books:

1. Experimental Microbiology, Plant Pathology and Biotechnology by K. R. Aneja

2. Refer books mentioned in theory syllabus

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