JNTU Question Papers Microbiology I Sem Nov 2008

JNTU Question Papers

Microbiology I Sem Nov 2008


1. What is a pure culture concept? Discuss the importance of pure culture methods

in development of microbiology. [16]


2. What are “Signature sequences” and Polymerase chain reaction? How are they

useful in classification of bacteria? [16]


3. Describe the general characteristics of Eubacteria. [16]


4. How is energy supplied to drive a conc. gradient across the membrane of the cell?



5. Describe the methodology for the enrichment of the following bacteria:

(a) Thiobacillus thiooxidans

(b) Rhizobium

(c) Halobacterium

(d) Clostridium. [4×4]


6. Define water activity. Explain how water activity influences the growth of different

microorganisms? [16]


7. Mention the most preferred method of sterilization for the following materials and

justify the selection of a particular method.

(a) Petridishes packed in a kraft paper

(b) Nutrient broth

(c) Heat sensitive Polythene bag

(d) Heat sensitive vitamin solution [4×4]


8. What are the indicators used for establishing the sterilization of materials by au-

toclaving or by dry heating in a Hot air oven?


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