GITAM University VII SEM Downstream Processing Syllabus
B.Tech. (BT) VII Semester
Course Code : EURBT 703 Category: CE
Credits: 3 Hours : 3 per week
Overview of bioseparation.
Recovery of intracellular products: Cell disruption methods – physical methods
(osmotic shock, grinding with abrasives, solid shear, liquid shear) – chemical methods
(alkali, detergents) – enzymatic methods.
Separation of cells and other insolubles from fermented broth – sedimentation,
filtration (pretreatment, filtration theory, continuous rotary filters), microfiltration,
Centrifugation (batch, continuous and basket).
Isolation and purification: precipitation, leaching, adsorption and ultrafiltration.
Precipitation (ammonium sulphate, organic solvents, high MW polymers)
Leaching: preparation of solid, steady and unsteady state operation, equipment,
analytical methods both theoretical and problematic approaches for single and
Liquid-liquid extraction methods in DSP.
Adsorption: Theory of adsorption. Industrial adsorbents, adsorption equilibria.
Freundlich equation. Single and multi-stage operations. Unsteady stae adsorpton.
Equipment for single stage and continuous contact. Ion-exchange. Column
Product polishing: crystallization and drying.
Crystallization: yields and material balances, heat effects and heat balances.
Equipment for crystallization. Crystallization theory: Rate of nucleation and rate of
crystal growth. Particle size distribution of crystals. Model for MSMPR.
Drying of bioproducts: methods of drying. Equipment for drying. Equilibrium
moisture content of bioproducts. Rate of drying curves. Convective, radiative and
conductive heat transfer in constant rate drying period. Falling rate drying period.
Freeze drying. Effect of thermal processing on food constituents.
Product recovery: ethanol, citric acid, penicillin, recombinant renin from E.coli.
1. Bioseparations: Downstream processing for biotechnology. P.A.Belter,
E.L.Cussler, W-S.Hu. Wiley-Interscience. (1988).
2. Bioseparation science and engineering. Harrison et al. 2006. Oxford Univ. Press.
3. Unit operations in chemical engineering.McCabe, Smith and Harriot. McGraw Hill
4. Separation Processes in Biotechnology. J.Asenjo.(1990)CRC.