GITAM University Syllabus Microbiology Syllabus
B.Tech. (BT) III Semester
Course Code: EURBT304 Category: BS
Credits: 3 Hours : 3 per week
History and development of Microbiology: Contributions of van Leeuwenhock,
Joseph Lister, Pasteur, Koch, Jenner, Winogradsky, Beijerinck.
Microbial taxonomy and diversity: Bacteria, Archea and their broad classification,
molecular approaches to microbial taxonomy. Physiology of Archaebacteria –
thermophiles, psychropiles, halophiles, methanogens.
Morphology and functions of Viruses, Yeast, Molds and Bacteria.
Viruses: Morphology of viruses – size, shape and symmetry, replication of viruses –
lytic and lysogenic cycle.
Yeasts and Molds: morphology, life cycle, economic importance of yeast and
Bacteria: ultrastructure of bacteria, cell wall, cell membrane, flagella, pili, capsule,
endospore, and cell inclusions, differences between prokaryotic and eukaryotic cell.
Microbial growth: bacterial growth batch, fed batch, continuous kinetics, synchronous
growth and methods of growth estimation, fungal growth.
Control of microorganisms, sterilization and disinfection, effect of physical (moist
and dry heat, radiation and filtration) and chemical agents, antibiotics: classification,
mode of action and resistance. Microbial nutrition: Nutrition requirements, nutritional
types of bacteria, uptake of nutrients by cell.
Methods in microbiology: culture media, synthetic and complex media, solidifying
agents, types of media, isolation of pure cultures – spread plate, pour plate and streak
plate, preservation of microorganisms, light(bright field only) and electron
Applied Microbiology: Water, Food and Milk borne contamination and remedy. Basic
microbial genetics – conjugation, transformation and transduction. Strain
improvement of microbes of industrial importance.
1. Microbiology: Prescott, Harley and Klein. 2008. 7th ed. McGraw Hill
2. Brock Biology of Microorganisms, Madigan, M.T., Martinko, JM and Parker,
2003. 10th ed. Prentice Hall.
3. Microbiology, Pelczar, M.J., Chan, ECS and Krieg, NR , 2007. 5th ed. Tata
5. General Microbiology, Stanier, R.Y.Ingraham, J.L., Wheelis, M.L. and Painter,
P.5th ed. 2003. The MacMillan Press Ltd.
6. Microbiology: Ananthanarayanan, R and J.Paniker, J. 2005 7th ed. Orient