GITAM University Bio analytical Techniques Syllabus
B.Tech. (BT) III Semester
Course Code : EURBT303 Category : BS
Credits: 3 Hours : 3 per week
Chromatography: General Principles. Modes of chromatography. Principles, chromatographic media and applications of the following types of chromatography: Ion-exchange, Gel permeation, Affinity, Gas-Liquid, HPLC. Electrophoresis: General principles. Polyacrylamide and agarose gel electrophoresis. Isoelectric focusing. 2DGE. Pulse field gel electrophoresis. Capillary electrophoresis. Centrifugation: Principles. Differential centrifugation. Density gradient
centrifugation. Analytical centrifugation – sedimentation velocity and sedimentation
equilibrium for determination of Mr. Radioisotope techniques: Principles, measurement and applications of radioactivity.
UV-Visible Spectroscopy: Principles, instrumentation and applications. Turbidimetry and Nephelometry: Principles and applications. Infrared and Raman: Principles, instrumentation and applications.
Spectrofluorimetry: Basic principles. Uses of Fluorescence parameters, intensity, wavelength dependence, quantum yield, lifetime, polarization and rate of resonance energy transfer with emphasis on DNA sequencing, Fluorescence Immunoassays, and Molecular beacons. Mass spectrometry: Basic Principles. Ionization techniques with emphasis on EI, FAB, Electrospray and MALDI. Analyzers with emphasis on Magnetic sector, quadrupole and TOF. FTICRMS. Applications of MS– determination of relative molecular mass, empirical formula, small molecule structural analysis, peptide sequencing and protein identification for proteomic studies.
ESR spectroscopy: Principles and applications.
NMR spectroscopy: Principles of magnetic resonance. Use of NMR parameters chemical shift, coupling constants and areas for structural elucidation. Nuclear Overhauser effect. Principles of FTNMR and 2D-NMR. Introduction to Protein structure determination by NMR. Principles of MRI and MR-spectroscopy.
Electrochemical methods: Principles of potentiometry. Clark’s oxygen electrode.
Biosensors: Principles and applications of electrochemical, thermometric, optical and
piezoelectric biosensors. Glucose biosensors.
Microarrays: Basic principles. Introduction to different types. Methods of manufacture. Applications – differential expression, SNP analysis.
1. Practical biochemistry. Principles and Techniques. Keith Wilson and John Walker.
1994. 5th ed. Cambridge University Press.
2. Biophysical chemistry. Principles and Techniques. Upadhyay, Upadhyay and Nath.
11th ed. Himalaya Publishing House.
1. Molecular biology and biotechnology. Walker and Rapley. (for Biosensors). 4th ed. 2003.
2. Microarrays for an integrative genomics. I.S.Kohane, Alvin Kho and Atul J.Butte.
2004. Ane books/MIT press.
3. NMR spectroscopy of proteins and nucleic acids. K.Wuthrich (for NMR spectroscopy)
4. Spectrometric identification of organic compounds. Silverstein, Webster and
Kiemle (for problems in NMR, MS) 6th ed. 2004.