AIIMS Microbiology Syllabus

AIIMS Microbiology Syllabus

The goal of teaching microbiology to undergraduate medical student is to provide an understanding of the infectious disease in order to deal with the etiology, pathogenesis, laboratory diagnosis, treatment and control of infections.

OBJECTIVES

(A)  Knowledge

At the end of the course, the student shall be able to:

  1. state the infective micro-organisms of the human body and describe the host parasite relationship
  2. list pathogenic micro-organisms and describe the pathogenesis of the diseases produced by them
  3. state or indicate the modes of transmission of pathogenic and opportunistic organisms and their sources, including insect vectors responsible for transmission of infection
  4. describe the mechanisms of immunity to infection
  5. acquire knowledge on suitable antimicrobial agents for treatment of infection and scope of immunotherapy and different vaccine available for prevention of communicable diseases
  6. apply methods of disinfection and sterilization to control and prevent hospital and community acquired infections
  7. recommend laboratory investigations regarding bacteriological examination of food, water, milk and air

(B)  Skills

At the end of the course, the student shall be able to :

  1. plan and interpret laboratory investigations for the diagnosis of infectious diseases and to correlate the clinical manifestations with the etiological agents
  2. identify the common infectious agents with the help of laboratory procedures and use antimicrobial sensitivity tests to select suitable antimicrobial agents
  3. use the correct method of collection, storage and transport of clinical material for microbiological investigations

COURSE CONTENT III           SEMESTER

  1. Introduction to Microbiology

(i)      Natural history of microbial diseases.

(ii)     Unique differentiating features of eukaryotes and prokaryotes

(iii)    Source and spread of microbes

(iv)   Rationale for classifying microbes into bacteria, fungi viruses, parasites.

  1. Introduction to Bacteriology

–          The nature of bacteria

–          Morphological differences

–          Growth requirement

–          Nomenclature and classification

–          Enumeration of bacteria responsible for certain primary diseases.

  1. Bacterial Staining and Cultivation

–          Microscopy : types and principles

–          Staining : principles

–          Media for growth / bacterial colony

–          Familiarization with materials used

  1. Common Tests for Bacterial identification

–          Various types of staining such as simple, diferential staining; different procedures of staining and their principles

–          Motility testing

–          Common sugar fermentation and other biochemical tests such as Catalase / Coagulase/ citrate utilization/ nitrate reduction / urease/ PPA/ OF/ Indole etc.

–          Sensitivity testing

  1. Introduction to parasitology

–          Biology of protozoa

–          Protozoan parasites causing human infection

–          Medically important helminths

–          Ectoparasites

  1. Introduction to Virology

–          The nature and properties of viruses

–          Classification of viruses

–          Morphology

  1. Laboratory Diagnosis of Viral Infection

–          Brief appraisal of pathogenicity of viruses

–           Culture methods

–           Cytopathic effects

–          Inclusion bodies

–          Animal inoculation

–           Serological test (CFT, HAI, neutralisation)

  1. Introduction to Mycology

–          Nature of fungi : basic structures and classification

–           Superficial mycoses

–           Subcutaneous mycosis

–           Systemic fungal infections with opportunistic mycosis

  1. Common Laboratory Methods for Diagnosis of Fungal Infections

(i)       KOH preparation with principles

(ii)     Lactophenal cotton blue preparation

(iii)   Negative staining and procedures

(iv)   Special staining and procedures

(v)     Culture of fungi

(vi)   Serodiagnosis

  1. Collection of Transport of Samples

–           Collection of clinical samples

–          Transport of various appropriate clinical samples.

–          Transport media

–          Description of container with contents or no contents.

–          Preliminary processing of clinical samples

  1. Host-Parasite relationship

–          Presence of normal flora

–          Enumeration and explanation of various host-parasite interaction

–          Mechanism of pathogenesis adhesion/ colonisation/ virulence and toxigenicity

–          Host response

–          Koch’s postulates

  1. Bacterial AND Viral Genetics

–           Structure and replication of bacterial DNA

–          Plasmids

–          Transfer of genetic materials

 

–           Mutations

–          Viral replication

–          Interactions among viruses (recombination, genetic reactivation, complementation etc).

–           Epidemiology of viral infection

–           Recombinant DNA technology

  1. Immunity to infection

–          Normal immune system

–          Innate Immunity

–          Antigens – presentation and association in immunity

–          Immunoglobulins and their role in immunity

–           Cell mediated immunity and their role

–           Hypersensitivity

–          Immunodeficiency

–           Tolerance

  1. Immunodiagnosis

–          Antigen-antibody reactions in infectious diseases and diagnostic tests based on these

  1. Vaccines
  2. Sterilisation and disinfection

–           Principles

–          Various methods

–           Demonstration of equipments and agents used in sterilization

–          Visit to CSSD

  1. Bacteriology of water and air Infections of Gastrointestinal Tract
    1. Microorganisms associated with gastrointestinal infections.

(Bacteria, parasites, viruses and fungi).

  1. Gastrointestinal infections caused by parasites 19a. Amoebiasis

–      Entamoeba spp

–     Naegleria spp

–     Acanthamoeba spp

19b. Amoebiasis (Micro, Gastro, Surg, Paeds)

19c. Other intestinal protozoal infections (Micro, Gastro, Paeds)

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