This book is designed as per syllabus of pharmacy council of India regulations as well syllabus of different universities. Viewing all aspects, book starts with spectrophotometric analysis and covers various aspects like colorimetry, flourimetry, flame photometry, nephloturbidimetry, chromatography (Thin layer chromatography, paper chromatography, column chromatography, high performance chromatography) and gas chromatography. The methodologies applied to various experiments are in forms of figures, diagrams for better understanding. The book contains calculations and questions for synopsis and viva to fulfill requirement of students.
This book Useful for B. Pharm Student.
Experiment No. 1: To study the determination of absorption maxima and the effects of solvents on absorption maxima of organic compounds.
Experiment No. 2: To perform an assay of Paracetamol tablet using the calibration curve method.
Experiment No. 3: To Estimate concentration of Ibuprofen and Paracetamol by using Simultaneous equation method. (Vierordt’s method).
Experiment No. 4: To estimate concentration of Paracetamol and Aceclofenac by Absorbance ratio Method (Q Analysis method).
Experiment No. 5: Estimation of dextrose in blood by colorimetry.
Experiment No. 6: Estimation of Sulfanilamide in blood by colorimetry.
Experiment No. 7: Estimation of Sulfanilamide in Urine by colorimetry.
Experiment No. 8: To find out the content of quinine sulphate by fluorimetry.
Experiment No. 9: To find out the content of Riboflavin by fluorimetry.
Experiment No. 10: To study effect of quenching on florescence of Quinine Sulfate.
Experiment No. 11: To determine the potassium ion concentration in the given sample of KCl (Potassium Chloride) by flame photometry.
Experiment No. 12: To determine the potassium ion concentration in the given sample of NaCl (Sodium chloride) by flame photometry.
Experiment No. 13: Determination of Sulphate by Nephelometry.
Experiment No. 14: Determination of Chlorides by Turbidimetry.
Experiment No. 15: To separate and determine Rf value for the given mixture of amino acid by Ascending Paper chromatography.
Experiment No. 16: To separate and determine Rf value for given mixture of amino acid by Thin layer chromatography.
Experiment No. 17: To separate and determine Rf value for given mixture of sugar by Thin layer chromatography.
Experiment No. 18: To separate and identify Curcumin (Plant pigment) from curcuma longa Rhizome extract by column chromatography.
Experiment No. 19: Demonstration of HPLC instrument.
Experiment No. 20: To perform a Demonstration of Gas chromatography.