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Techniques And Methods In Biology

By K L Ghatak more
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Product Specifications

Publisher PHI Learning
ISBN 9788120341715
Author: K L Ghatak
Number of Pages 696
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About The Book Techniques And Methods In Biology
Book Summary:

This comprehensive and accessible text discusses all the topics prescribed for the students of Life Sciences taking the National Eligibility Test (NET). Besides, the book would also be useful for undergraduate and postgraduate students of Biotechnology, and postgraduate students of Botany and Zoology.

The book discusses spectroscopy which forms the core of modern research, be it physical sciences or life sciences, and microscopy, which is now an indispensable analytical tool in Biological Science, with all its different forms. It also illustrates radioactivity and related phenomena so as to justify their widespread applications in modern biological, medical and chemical researches. The book evaluates the role of statistics in biological as well as physiological/medical phenomena, and systematically analyses electrophysiological methods, histochemical and immuno techniques, and molecular biology.


Questions and their answers are interspersed throughout the text so as to make the discussion clear and meaningful.

Use of mathematical calculations and formulas is kept to a minimum.

Table of Contents:
Preface Acknowledgements
Part I: Biophysical Methods
1. Spectroscopy
2. Infrared Spectroscopy
3. Ultraviolet and Visible Spectroscopy
4. Colorimetry and Visible Spectrophotometry
5. Fluorescence and Phosphorescence Spectrophotometry
6. Optical Rotatory Dispersion and Circular Dichroism
7. Nuclear Magnetic Resonance Spectroscopy
8. Electron Spin Resonance Spectroscopy
9. Atomic Absorption Spectroscopy
10. X-ray Crystallography/Diffraction
11. Mass Spectrometry
12. Chromatography
Part II: Microscopy
13. Electron Microscopy
14. Optical/light Microscopy
15. Fixation and Staining
16. Phase Contrast Microscopy
Part III: Radioactivity
17. Radio isotopes in Analytical Techniques
18. Tracer and Tracer Techniques
19. Biological Effects of Radiation
20. Autoradiography
21. Radiation Dosimetry
Part IV: Statistical Methods
22. Statistical Methods
Part V: Electrophysiological Methods
23. Electrophysiological Methods
Part VI: Histochemical and Immunotechniques
24. Flowcytometry and Cytophotometry
25. Antibody and Immunochemistry
Part VII: Molecular Biology and Recombinant DNA Methods
26. Electrophoresis and Electrofocusing
27. Centrifugation
28. Nucleic Acid Hybridization and Cot Curves
29. Polymerase Chain Reaction
30. Sequencing of Proteins
31. Nucleic Acid Sequencing
32. Strategies for Genome Sequencing and Cloning
33. Polymorphism
34. Library Generation
35. Mutagenesis
36. Carbohydrates
37. Gene Expression
38. Microarray Analysis of Gene Expression
39. Gene and Gene Knock Out
40. Recombinant Protein Expression
41. Lipids

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