VTU Syllabus Biotechnology Engineering 4th Semester

VTU Syllabus Biotechnology Engineering 4th Semester

VTU Syllabus Biotechnology Engineering 4th Semester

VTU Syllabus Biotechnology Engineering 4th Semester: To prepare the fourth-semester exam, you must aware of the latest VTU Syllabus Biotechnology Engineering 4th Semester and marking scheme. With the latest VTU Syllabus, Biotechnology Engineering 4th Semester students get to know the important chapters and concepts to be covered in all subjects.

In the depth knowledge in every topic of Biotechnology Engineering 4th Semester will also helpful to crack the various competitive exams like Gate.

Here we are providing you the complete guide on VTU Syllabus Biotechnology Engineering 4th Semester 2020 and Marking Scheme.

VTU Syllabus Biotechnology Engineering 4th Semester 2020

With the latest Biotechnology Engineering Syllabus for the 4th Semester, you can create a solid study plan and score a better mark in all subjects in the semester exam.

You must have Biotechnology 4th Semester books & study materials, Previous years questions paper along with the latest Biotechnology 4th sem Syllabus to enhance your semester exam preparation.

Before starting the complete guide on VTU Syllabus Biotechnology Engineering 4th Semester 2020, let’s check the highlights of VTU from the table below.

VTU Belgaum Highlights

Established year 1998
Approvals AICTE, UGC, COA( Council of Architecture)
Courses UG(35), PG(94), Ph.D & Research(592 departments) Quality Improvement Program(13)
Official website www.vtu.ac.in
Number of Students +325000
Collaborations Bosch Rexroth AG-Germany

Virginia Commonwealth University

University of California

Deshpande Foundation-Startup Center

India Electronics and Semiconductor Association

IBM India Ltd. Bengaluru

Intel Asia. Bengaluru

Check the latest VTU Syllabus Biotechnology Engineering 4th Semester from below.

STOICHIOMETRY

Course Code 18BT41 CIE Marks 40
Teaching Hours/Week (L:T:P) (3:1:0) SEE Marks 60
Credits 03 Exam Hours 03
Course Learning Objectives:

· To learn fundamentals of chemical calculations and material and energy balance.

· To discuss the material balance aspects involving chemical reactions and without chemical reactions.

· To highlight the energy balance and material balance for the development of bioprocess technology

Module-1
BASIC CHEMICAL CALCULATIONS AND MATERIAL BALANCE

Concept of atom and mole, expressing composition of mixtures in Solids, liquids and gases. Expressing composition of mixtures and solutions – Percentage by weight percentage, mole percentage and

Volume percentage; Normality, Morality, Molality. Generalized material balance equations for distillation, absorption, extraction, crystallization, mixing, drying & evaporation

Module-2
MATERIAL BALANCE WITHOUT CHEMICAL REACTIONS AND FUELS

Material balances calculation in Distillation, Absorption, Extraction, Crystallization, Drying, Mixing and Evaporation Operations, Fuels – types of fuels, (solid, liquid and gaseous fuel), relevance to biofuels, characteristics of fuels, Ultimate and proximate analyses of fuels

Module-3
MATERIAL BALANCE INVOLVING CHEMICAL REACTIONS

Material balances calculation involving bypass, recycle and operations. Generalized material balance equations, Principles of stoichiometry, Definitions of limiting and excess reactants, and percentage conversion, yield and percentage yield, Selectivity, and problems relating to these unit processes

Module-4
ENERGY BALANCE

General energy balance equation for steady state. Heat capacity, estimation of heat capacity for solids,

liquids, gases and their mixtures. Enthalpy, Standard Heat of formation, standard heat of reaction, Standard heat of combustion, Calculation of heat of reaction at elevated temperature.

Module-5
BIOPROCESS PRINCIPLES & STOICHIOMETRY OF BIOPROCESS

Historical development of bioprocess technology; Bioprocess principles and operations, generalized process flow sheets. General material balance equation for steady state (for manufacture of penicillin and ethanol) – outline of a bioprocess and the various (upstream and downstream) unit operations involved in bioprocesses.

Course Outcomes: At the end of the course the student will be able to:

· Discuss the significance of material and energy balance for bioprocess technology.

· Solve problems related to material and energy balance to give solutions for bioprocess development.

· Develop the flow-sheet for general processes operating in bioprocess industry.

· Apply the stoichiometry of microbial growth and product formation involved in bioprocess technology

Question paper pattern:

· The question paper will have ten full questions carrying equal marks.

· Each full question will be for 20 marks.

· There will be two full questions (with a maximum of four sub- questions) from each module.

· Each full question will have sub- question covering all the topics under a module.

· The students will have to answer five full questions, selecting one full question from each module.

Sl No Title of the Book Name of the Author/s Name of the Publisher Edition and Year
Textbooks
1 Principles of Biochemistry Albert L. Lehninger, David L. Nelson, and

Michael M. Cox

W.H. freeman and company 1970
2 Bioprocess Engineering Principles Pauline Doran Academic Press 2nd Edition 2012
3 Biochemical Engg. Fundamentals J E Bailey & D.

F. Ollis

McGraw Hill 1986
4 Biochemical Calculations I.H.Segel John Wiley & Sons 1976
Reference Books
1 Basic Principles and Calculations

in Chemical Engineering

David

Himmelblau

PHI Eighth Edition

2012

2 Bioprocess Engineering Shule and

Kargi,

Prentice Hall 3rd Edition 2017
3 Chemical Process Calculations R. Asokan University Press 2011
Web links and Video Lectures:

MOLECULAR BIOLOGY

Course Code 18BT42 CIE Marks 40
Teaching Hours/Week (L:T:P) (4:0:0) SEE Marks 60
Credits 04 Exam Hours 03
Course Learning Objectives:

· To familiarize with the cell and molecular biology of both Prokaryotes and Eukaryotes.

· To acquire basic fundamental knowledge and explore skills in molecular biology

· To become aware of the complexity and harmony of the cells.

· To learn the molecular mechanism of DNA replication, repair, transcription, protein synthesis and gene

regulation in various organisms.

Module-1
INTRODUCTION & REPLICATION OF DNA:

Chromosomal theory of heredity, genes and their location. Genetic code, Information flow in biological systems: central dogma, updated central dogma. Structures and forms of nucleic acids – DNA and RNA. Reversible denaturation and hyperchromic effect. Tertiary structure of DNA: DNA supercoiling. Replication of DNA, structure and function of DNA polymerases, models of replications in prokaryotes, mechanism of DNA replication and enzymes involved. Proteomics of DNA replication, Fidelity of DNA replication, Inhibitors of

DNA replication

Module-2
TRANSCRIPTION:

Structure and function of RNA polymerases (prokaryotes & eukaryotes), mechanism of transcription in

prokaryotes and eukaryotes, transcription factors, Fidelity of RNA synthesis, post-transcriptional processing (RNA editing, siRNA, splicing, poly A tail and 5’ capping), Ribozymes, transcription inhibitors.

Module-3
TRANSLATION:

Mechanism of translation, activation of amino acid initiation, elongation and termination of protein synthesis. Post-translational modification and protein targeting, protein splicing. Differences between prokaryotic and

eukaryotic protein synthesis, inhibitors of translation.

Module-4
GENE EXPRESSION IN PROKARYOTES & EUKARYOTES:

Regulation of gene expression in prokaryotes: Operon model, gal, lac, trp Operons; positive versus negative regulation. Regulation of eukaryotic gene expression, transcriptional control, homeobox in the control of

developments in insects and vertebrates.

Module-5
GENETIC RECOMBINATION, MUTATION & GENE MAPPING:

Genetic recombination in bacteria and viruses, site specific recombination, transposons and insertion sequences;

Retroviruses. DNA damage & Repair, Mutation, Role of recombination and transposition in evolution; gene mapping techniques.

Course Outcomes: At the end of the course the student will be able to:

· Explain replication, transcription and translation processes with underlying differences in prokaryotic and eukaryotic systems.

· Elaborate importance of genetic recombination with special reference to bacterial system.

· Outline DNA damage and repair mechanisms

Question paper pattern:

· The question paper will have ten full questions carrying equal marks.

· Each full question will be for 20 marks.

· There will be two full questions (with a maximum of four sub- questions) from each module.

· Each full question will have sub- question covering all the topics under a module.

· The students will have to answer five full questions, selecting one full question from each module.

Sl No Title of the Book Name of the Author/s Name of the Publisher Edition and Year
Textbook/s
1 Essentials of Molecular Biology David Freifelder Narosa Pub. House 1990
2 Molecular Biology of the Cell Alberts et al Garland Publishing 2002.
3 Molecular Biology of the

Gene

James D

Watson et al

Pearson Education Seventh Edition

2017

Reference Books
1 Principles of Gene

manipulation and Genomics

Primrose, Oxford University Press 2006

IMMUNOTECHNOLOGY

Course Code 18BT43 CIE Marks 40
Teaching Hours/Week (L:T:P) (3:0:0) SEE Marks 60
Credits 03 Exam Hours 03
Course Learning Objectives:

· Learn the underlying concepts of molecular and cellular mechanisms involved in the development and regulation of the immune response

· Describe the cause and treatment for Immune System Pathologies and Dysfunctions.

· Learn the important techniques of Immunodiagnosis.

Module-1
IMMUNE SYSTEM :

Cells and organs of immune system, Process of hematopoiesis and role of each cells, primary and secondary lymphoid organs, innate and acquired immunity, Humoral and Cell mediated immunity. Antigens: Chemical and biological Factors affecting antigenicity/Immunogenicity and molecular nature, Haptens, adjuvants, Antibodies: their structure and function, Immunoglobulin classes and subclasses (isotypic, allotypes, idiotypes

and anti-idiotytopic antibodies).

Module-2
HUMORAL AND CELL MEDIATED IMMUNITY:

B-lymphocytes and their activation, Class switching mechanism , antibody genes and generation of diversity, production of monoclonal antibodies, polyclonal antibodies and applications, cytokines, Thymus derived lymphocytes (T cells) – their ontogeny and types, Activation of T-cells, Major histocompatibility Complex

(MHC) Complex – MHC Class I and II molecules. Antigen processing and presentation process.

Module-3
IMMUNE SYSTEM IN HEALTH AND DISEASE:

Complement system and its pathways, Gell and Coombs classification of Hypersensitivity reactions and Diagnosis and treatment. Autoimmune disorders, types, animal model and treatment. Immune response to infections: immunity to viruses, bacteria, fungi and parasites, Immunodeficiency disorders: Primary and secondary (AIDS). Injury and inflammation, Vaccines and their types, classification and immunization

schedule

Module-4
TRANSPLANTATION AND TUMOR IMMUNOLOGY:

Transplantation and its classification, Immunologic basis of graft rejection and its mechanism , Transplantation antigens, tissue typing role of MHC molecules in allograft rejection, Clinical transplantations, bone marrow, HSC transplantation and immune suppressive therapy. Tumors of the immune system, tumor antigens and

immune response to tumors, tumor immunotherapy.

Module-5
MOLECULAR IMMUNOLOGY & IMMUNODIAGNOSIS

Antigen antibody interaction – Precipitation reactions, Agglutination reactions, ABO Blood typing principles. Principles and applications of ELISA, Radio Immuno Assay (RIA), western blot analysis, immuno- electrophoresis, Immunofluorescence, chemiluminescence assay, fluorescence activated cell sorting (FACS) analysis. Role of stem cells technology in immunology, Production of humanized monoclonal antibodies

(Single chain fragment variable), immunotherapy with genetically engineered antibodies,

Course Outcomes:

At the end of the course the student will be able to:

· Outline the molecular and cellular mechanisms involved in the development and regulation of the immune response,

· Describe the cause, challenges and treatment for Immune System Pathologies and Dysfunctions.

· Apply the major immunological laboratory techniques and their application to both clinical analysis and experimental research

Question paper pattern:

· The question paper will have ten full questions carrying equal marks.

· Each full question will be for 20 marks.

· There will be two full questions (with a maximum of four sub- questions) from each module.

· Each full question will have sub- question covering all the topics under a module.

· The students will have to answer five full questions, selecting one full question from each module.

Sl No Title of the Book Name of the Author/s Name of the Publisher Edition and Year
Textbook/s
1 Immunology – an Introduction Tizard Thomson. 1984
2 Immunology

&Immunotechnology

Ashim K

Chakravarthy

Oxford University Press 2006
3 Immundiagnostics S C Rastogi New Age International 1996
Reference Books
1 Essential Immunology Roitt I.

Blackwell

Scientific Publications,

Oxford

13th Edition

2017

2 Immunology: A Short Course Richard

Coico, Geoffrey Sunshine

Wiley-Blackwell 7th Edition 2015
3 Understanding Immunology Peter Wood Pearson Education 2001

CELL CULTURE TECHNIQUES

Course Code 18BT44 CIE Marks 40
Teaching Hours/Week (L:T:P) (3:0:0) SEE Marks 60
Credits 03 Exam Hours 03
Course Learning Objectives:

· To learn the basic structure ,organization ,scope and function of Biomolecules

· To learn the transformation of energy within the living organisms

· To learn the composition, structure and function of biological membranes

· To learn the types and functions of transport system

Module-1
CELL CULTURE LABORATORY DESIGN AND EQUIPMENTS:

Planning, construction and services; Layout; Sterile handling area; Incubation; Hot room; Air circulation; Service bench; Laminar flow; Sterilizer; Incubators; CO2 incubator; Culture Racks, Colony Counters, Refrigerators and freezers; Centrifuge; Inverted stage microscope; Magnetic stirrer; Liquid nitrogen freezers; Slow cooling system for cell freezing; Water bath; Autoclaves and hot air oven; Pipette washers; Water purification system; Fluid handling systems and other equipments; Washing, packing and sterilization of different materials used in plants, animals and microbial cell cultures; Aseptic concepts; Maintenance of sterility; Cell culture vessels

Module-2
MEDIA AND REAGENTS :

Types of cell culture media for plants, animals and microbial cells; Ingredients of media; Physiochemical properties; Buffers; Oxygen; Osmolarity; Temperature; Balance salt solutions; Antibiotics, growth supplements; Conditioned media; Other cell culture reagents; Preparation and sterilization of cell culture media and other reagents.

Module-3
ANIMAL CELL CULTURES TECHNIQUES:

History of animal cell culture; Different tissue culture techniques; Cell separation, disaggregation of the explants, mechanical and enzymatic disaggregation; Continuous cell lines; Organ culture, techniques, advantages, disadvantages, applications; Cell cultures, substrate culture and suspension culture; Primary cell culture; Secondary cell culture (cell lines); Development, characterization and maintenance of cell lines, Cryopreservation; Commercial scale production of animal cells; stem cells- fate mapping, application; Application of animal cell culture for in vitro testing of drugs;

Module-4
PLANT CELL CULTURE TECHNIQUES:

Cellular Totipotency, And its Applications. Organogenesis, factors affecting organogenesis. Cyto- differentiation. Somatic Embryogenesis, Synthetic Seeds, Techniques for production of haploids, diploidization, production of double haploids and their Applications. Triploids production – Endosperm culture and Applications. Secondary metabolite production, selection of high yielding lines, elicitation, immobilization of cultures, hairy root culture and biotransformation. Factors affecting secondary metabolites, industrial application of secondary metabolites. Molecular farming.

Module-5
MICROBIAL CELL CULTURE TECHNIQUES:

Auxotroph isolation – replica plating technique, Screening Preservation of microbial products. Production of antibiotics. Enumeration and screening of novel microbial secondary metabolites, strain improvement, Use of microbes in industrial waste treatment. Microbial leaching.

Course Outcomes:

At the end of the course the student will be able to:

· Differentiate between the various sources of cells to be used in cell culture techniques

· Correlate between different biological samples and understand the importance of different media in tissue culture

· Comprehend the applications of plant, animal and microbial cell culture in industry, healthcare and environment.

Question paper pattern:

· The question paper will have ten full questions carrying equal marks.

· Each full question will be for 20 marks.

· There will be two full questions (with a maximum of four sub- questions) from each module.

· Each full question will have sub- question covering all the topics under a module.

· The students will have to answer five full questions, selecting one full question from each module.

Sl No Title of the Book Name of the Author/s Name of the Publisher Edition and Year
Textbook/s
1 Plant Cell Culture: A Practical

Approach

R.A. Dixon &

Gonzales

IRL Press 1994
2 Culture of animal cells-A manual of basic technique and specialized

applications

R. Ian Freshney Wiley Blackwell publishers 1983
3 Microbial Biotechnology Alexander N Glazer, Hiroshi

Nikaido

W H Freeman & Company 1995
Reference Books
1 Living resources for

Biotechnology, Animal cells

Doyle, R. Hay

and B.E. Kirsop

Cambridge University Press 1990
2 Plant Tissue Culture Sathyanarayana

B N,

IK Intl. Publishers 2007
3 Principle of Microbe & Cell

Cultivation

SJ Prit Blackwell Scientific co 1975

BIOCHEMICAL THERMODYNAMICS

Course Code 18BT45 CIE Marks 40
Teaching Hours/Week (L:T:P) (3:1:0) SEE Marks 60
Credits 03 Exam Hours 03
Course Learning Objectives:

· To know the basic concepts of thermodynamics in process industry.

· To understand the significance of zeroth, I, II & III laws of thermodynamics.

· To be aware of concepts of thermodynamic properties of fluids & demonstrate various equations of state & their applications.

· To illustrate the importance of partial molar properties & the concepts of phase equilibrium.

Module-1
BASIC CONCEPTS & LAWS OF THERMODYNAMICS: System, Surrounding & Processes, Closed and Open systems, State Properties, Intensive &Extensive Properties State and Path functions, Equilibriumstate,enthalpy,specific heat,Reversible and Irreversible processes. Zeroth law of Thermodynamics, General statement of First law of Thermodynamics, First law for Cyclic Process, Non- Flow Process, Flow process, Heat capacity. Heat reservoir and Heat engines. General statements of the second law, Concept of entropy, Carnot principle, Calculation of entropy changes, Third law of Thermodynamics. Numericals.
Module-2
PVT BEHAVIOUR AND COMPRESSIBILITY CHARTS:

PVT Behavior of pure fluids, equations of state & ideal gas law, Processes involving ideal gas law: Constant volume, constant pressure, constant temperature, adiabatic & polytrophic processes, Equations of state for real gases: Vander Waals equation, Redlich-Kwong equation, Peng-Robinson equation, virial equation. Numericals. Principles of corresponding states, generalized compressibility charts, Heat effects accompanying chemical reactions, Standard heat of reaction, formation, combustion, Hess’s law of constant

heat summation, effect of temperature on standard heat of reaction. Numericals.

Module-3
PROPERTIES OF PURE FLUIDS :

Reference properties, energy properties, derived properties, work function, Helmholtz free energy, Gibbs free energy, Relationships among thermodynamic Properties: Exact differential equations, fundamental property relations, Maxwell’s equations, Clapeyron equations, modified equations for internal energy (U) & enthalpy (H), Effect of temperature on U, H & Entropy (S). Gibbs-Helmholtz equation. Concept of Fugacity, Fugacity

coefficient, effect of temperature and pressure on fugacity, Determination of fugacity of pure gases, solids and liquids, Activity: Effect of temperature and pressure on activity. Numericals

Module-4
PROPERTIES OF SOLUTIONS & PHASE EQUILIBRIA:

Partial molar properties of solution and its determination , chemical potential –effect of temperature and pressure , lewis –randall rule, Raoults law for ideal solutions, fugacity in solutions, Henry’s law and dilute solutions – ideal behavior of real solutions and Henry’s law, Activity in solutions, Activity coefficients – effect of temperature and pressure, Gibbs – Duhem equation, calculation of activity coefficients using Gibbs- Duhem equation. Numericals.

Criteria of phase Equilibria, criterion of stability, Duhem’s theorem, Vapour-Liquid Equilibra in ideal and non-Ideal solutions, Azeotropes. Numericals.

Module-5
BIOCHEMICAL ENERGETICS:

Coupled reactions and energy rise compounds, Reaction Stoichiometry, criteria of biochemical reaction equilibrium, equilibrium constant and standard free energy change, effect of temperature, pressure on equilibrium constants and other- factors affecting equilibrium conversion – Le – chatelier’s principle, liquid phase reactions, heterogeneous bioreactionequilibria, phase rule for reacting systems, Liquid-Liquid Equilibrium diagrams. Numericals.

Course Outcomes:

At the end of the course the student will be able to:

· Describe the concepts of system, surrounding, process, laws of thermodynamics & entropy.

· Explain the PVT behaviour of pure fluids & gases & derive equations of state for real gases.

· Distinguish between work function, Gibbs free energy

· Determine the partial molar properties, activity coefficients of the solution.

· Illustrate the phase rule for reacting systems and effect of temperature, pressure on equilibrium

constants

Question paper pattern:

· The question paper will have ten full questions carrying equal marks.

· Each full question will be for 20 marks.

· There will be two full questions (with a maximum of four sub- questions) from each module.

· Each full question will have sub- question covering all the topics under a module.

· The students will have to answer five full questions, selecting one full question from each module.

Sl No Title of the Book Name of the Author/s Name of the Publisher Edition and Year
Textbook/s
1 Introduction to Chemical Engineering thermodynamics J.M. Smith ,

H.C. Van Ness &M.M.Abbott

MGH. 6th Ed (2003)
2 Biochemical Calculations Irwin H.Segel John Wiley & Sons 2nd Ed,( 1976)
3 Engineering Thermodynamics R K Singal,

MridualSingal

I K Intl. 2010
Reference Books
1 Chemical Engineering

Thermodynamics

Y.V.C. Rao New Age International 1997
2 A Textbook of Chemical

Engineering Thermodynamics

K.V. Narayanan PHI 1st Ed (2001)

CLINICAL BIOCHEMISTRY

Course Code 18BT46 CIE Marks 40
Teaching Hours/Week (L:T:P) (3:0:0) SEE Marks 60
Credits 03 Exam Hours 03
Course Learning Objectives:

· Learn the metabolic pathways of bio-molecules occurring at cellular and molecular level in living organisms.

· To learn basic principles of biochemistry and pathophysiology associated with metabolism.

· To apply the concepts in the clinical biochemistry aspects.

· Gain insight into the clinical manifestations of various metabolic disorders

Module-1
METABOLISM OF CARBOHYDRATES AND LIPIDS:

Glycolysis–metabolism. Aerobic and anaerobic pathway and regulation, TCA cycle, NADPH Cycle, Glyoxylate cycle, Pentose Phosphate Pathway. Electron transport chain and oxidative phosphorylation, energy balance sheet. Gluconeogenesis – regulation of gluconeogenesis. Biosynthesis of polysaccharides. Biosynthesis of fatty acids, cholesterol, phospholipids, glycolipids. Biodegradation of triglycerides and fatty

acids.

Module-2
DISORDERS OF CARBOHYDRATE METABOLISM:

Diabetes mellitus, glycohemoglobins, hypo-glycemias, galactosemia and ketone bodies. Various types of glucose tolerance tests. Glycogen storage diseases. Physiology of lipids/lipoproteins. Lipidosis. Clinical inter-

relationships of lipids (sphingolipidosis and multiple sclerosis), lipoproteins and apolipoproteins. Diagnostic tests for HDL-cholesterol, LDL-cholesterol and triglyceride disorders.

Module-3
METABOLISM OF AMINO ACIDS AND NUCLEIC ACIDS:

Biosynthesis and catabolism of essential amino acids: Lysine, Phenylalanine and Glutamine. Deamination,

transamination and urea cycle. Metabolism and regulation of Purines, pyrimidine and precursors of nucleic acids (nucleosides & nucleotides).

Module-4
DISORDERS OF AMINO ACIDS AND NUCLEIC ACIDS METABOLISM AND HORMONAL DISTURBANCES:

a) Disorders of amino acid metabolism – Phenylalanemia, homocystinuria, tyrosinemia, MSUD, phenylketonuria, alkaptonuria, albinism and animoacidurias. b) Disorders of nucleic acid metabolism- Disorders in purine/ pyrimidine metabolism. c) Protein hormones (anterior pituitary hormones, posterior pituitary hormones), steroid hormones, adrenocorticosteroids, and reproductive endocrinology. Disturbances in

thyroid function.

Module-5
DISORDERS OF ACID-BASE BALANCE AND THEIR RESPIRATORY AND RENAL MECHANISMS:

Evaluation of organ function tests, Assessment and clinical manifestations of renal, hepatic, pancreatic, gastric and intestinal functions. Clinical importance of bilirubin. Diagnostic enzymes: Principles of diagnostic enzymology. Clinical significance of aspartate aminotransferase, alanine aminotransferase, creatine kinase, aldolase and lactate dehydrogenase. Enzyme tests in determination of myocardial infarction. Enzymes of

pancreatic origin and biliary tract.

Course Outcomes:

At the end of the course the student will be able to:

· Understand the basic metabolic pathways it’s energetics and regulations of biomolecules.

· Discuss the biochemistry and pathophysiology associated with metabolism.

· Apply the theoretical concepts of biochemistry in diagnosis of biological samples.

· Assess the clinical manifestations of various metabolic disorders.

Question paper pattern:

· The question paper will have ten full questions carrying equal marks.

· Each full question will be for 20 marks.

· There will be two full questions (with a maximum of four sub- questions) from each module.

· Each full question will have sub- question covering all the topics under a module.

· The students will have to answer five full questions, selecting one full question from each module.

Sl No Title of the Book Name of the Author/s Name of the Publisher Edition and Year
Textbook/s
1 Clinical Biochemistry Allan Gaw, Michael Murphy, Robert Cowan, Denis O’Reilly, Michael Stewart and James

Shepherd

Churchill Livingstone 4thEdn., 2008
2 Medical biochemistry N V Bhagavan Academic Press 4thEdn., 2001
Reference Books
3 Lecture Notes: Clinical Biochemistry Geoffrey Beckett,

Simon Walker, Peter Rae, Peter Ashby

John Wiley & Sons 7th Edn, 2010
4 Textbook of Medical Biochemistry MN Chatterjea and RanaShinde Jaypee Brothers Eighth Edition 2011
5 Clinical Biochemistry Richard Luxton Scion Publishing Ltd. Second edition

2008

BIOCHEMISTRY LABORATORY

Course Code 18BTL47 CIE Marks 40
Teaching Hours/Week (L:T:P) (0:2:2) SEE Marks 60
Credits 02 Exam Hours 03
Course Learning Objectives:

· To know the basic laboratory mathematics.

· To analyze the concentration of unknown sample and interpret the results.

· To acquire means to manage experiments independently.

Sl. NO Experiments
1 pH measurements, volume / weight measurements, concentration units, sensitivity. Specificity, precision,

accuracy, preparation of buffers of constant strength.

2 Titration of amino acids with acids & bases.
3 Qualitative tests for carbohydrate and lipids.
4 Qualitative tests for amino acids and proteins.
5 Estimation of blood sugar by Folin method and by O-toluene method.
6 Estimation of inorganic phosphate by Fiske-Subbarao method.
7 Estimation of amino acid by Ninhydrin method.
8 Estimation of total cholesterol from Serum.
9 Determination of saponification value and iodine value of lipids with error analysis.
10 Determination of acetyl value of a lipid with error analysis.
11 Estimation of urea by diacetylmonooxime method with error analysis.
12 Estimation of iron from hemoglobin with error analysis.
13 Estimation of Vitamin C by titrimetric method
14 Estmation of lactose in milk by DNS method
15 Estimation of uric acid in blood by Caraway’s method
16 Estimation of creatinine in serum and urine by Jaffe’s method
Note: Minimum 12 experiments are to be conducted
Course Outcomes:

At the end of the course the student will be able to:

· Demonstrate the basic laboratory mathematics necessary to perform tests, make dilutions, and prepare buffer solutions.

· Compare/contrast Qualitative and quantitative analysis of various Biomolecules.

Conduct of Practical Examination:

1. All laboratory experiments are to be included for practical examination.

2. Breakup of marks and the instructions printed on the cover page of answer script to be strictly adhered by the examiners.

3. Students can pick one experiment from the questions lot prepared by the examiners.

4. Change of experiment is allowed only once and 15% Marks allotted to the procedure part to be made zero.∎

Reference Books:

1. Modern Experimental Biochemistry by Rodney Boyer, Pearson Education.

2. Practical Biochemistry by Cole, Cambridge University Press.

3. Lab Ref by Jaine Roskams & Linda Rodgers, IK Intl. Pub. House.

4. Manual of Practical Biochemistry for medical students, 2nd edition, University Press

IMMUNOTECHNOLOGY LABORATORY

Course Code 18BTL48 CIE Marks 40
Teaching Hours/Week (L:T:P) (0:2:2) SEE Marks 60
Credits 02 Exam Hours 03
Course Learning Objectives:

· Learn and demonstrate the various Cell biology and Immunodiagnostic techniques

· Independently carry out research experiments in immunology and cell biology

Sl. No Experiments
1 Agglutination Technique: ABO typing
2 Differential counting of WBC
3 Isolation of lymphocytes from peripheral blood
4 Bacterial Agglutination reaction-Widal test (Tube / slide agglutination)
5 Radial Immunodiffusion (RID)
6 Ouchterlony Double Diffusion (ODD)
7 Rocket immunoelectrophoresis (RIEP)
8 Counter-current immunoelectrophoresis (CCIEP)
9 Dot ELISA
10 Coombs test
11 Complement fixation test
12 Isolation of Chicken Immunoglobulin (IgY) from Egg Yolk
13 Immunoglobulin purification from serum by IEC
14 Western blotting
Note: Minimum 12 experiments are to be conducted
Course Outcomes:

At the end of the course the student will be able to:

· Learn and demonstrate the various Immunodiagnostic techniques like agglutination, precipitation, immunoelectrophoresis, qualitative analysis, ELISA, separation of Lymphocytes and Immunoblot

· Independently carry out research experiments in immunology and cell biology.

Conduct of Practical Examination:

1. All laboratory experiments are to be included for practical examination.

2. Breakup of marks and the instructions printed on the cover page of answer script to be strictly adhered by the examiners.

3. Students can pick one experiment from the questions lot prepared by the examiners.

4. Change of experiment is allowed only once and 15% Marks allotted to the procedure part to be made zero.∎

Reference Books:

1. Immunology by Kuby Cambridge University Press.

2. Immunology Laboratory Practices by Stryers

3. The Cell by Alberts Cambridge University Press

ADDITIONAL MATHEMATICS – II

(Mandatory Learning Course: Common to All Branches)

(A Bridge course for Lateral Entry students under Diploma quota to BE/B. Tech. programmes)

Course Code 18MATDIP41 CIE Marks 40
Teaching Hours/Week (L:T:P) (2:1:0) SEE Marks 60
Credits 00 Exam Hours 03
Course Learning Objectives:

· To provide essential concepts of linear algebra, introductory concepts of second & higher order differential equations along with methods to solve them,

· To solve Laplace & inverse Laplace transforms and elementary probability theory.

Module-1
Linear Algebra: Introduction – rank of matrix by elementary row operations – Echelon form. Consistency of system of linear equations – Gauss elimination method. Eigen values and eigen vectors of a square matrix. Application of Cayley-Hamilton theorem (without proof) to compute the inverse of a matrix-Examples.
Module-2
Higher order ODE’s: Linear differential equations of second and higher order equations with constant coefficients. Homogeneous /non-homogeneous equations. Inverse differential operator method for f(D)y=R(x) where R(x)= e ax, sin(ax), cos(ax), and polynomial in x only. Method of undetermined coefficients and

variation of parameters.

Module-3
Laplace transforms: Laplace transforms of elementary functions. Transforms of derivatives and integrals, transforms of periodic function and unit step function-Problems only.
Module-4
Inverse Laplace transforms: Definition of inverse Laplace transforms. Evaluation of inverse transforms by standard methods. Application to solutions of Linear differential equations
Module-5
Probability: Introduction. Sample space and events. Axioms of probability. Addition and multiplication theorems. Conditional probability – illustrative examples. Bayes’s theorem-examples.
Course Outcomes: At the end of the course the student will be able to:

· Use matrix theory for solving systems of linear equations in the different areas of linear algebra.

· Solve second and higher order differential equations occurring in of electrical circuits, damped/un- damped vibrations.

· Learn the Laplace transforms of standard and periodic functions.

· Utilize the inverse Laplace transforms to determine general or complete solutions to linear ODE.

· Explore the basic concepts of elementary probability theory and, apply the same to the problems of decision theory, synthesis and optimization of digital circuits.

Question paper pattern:

· The question paper will have ten full questions carrying equal marks.

· Each full question will be for 20 marks.

· There will be two full questions (with a maximum of four sub- questions) from each module.

· Each full question will have sub- question covering all the topics under a module.

· The students will have to answer five full questions, selecting one full question from each module.

Sl No

Title of the Book

Name of the Author/s

Name of the

Edition and Year

Textbook
1 Higher Engineering Mathematics, B.S. Grewal Khanna Publishers, 43rd Ed., 2015.
Reference Books
1 Advanced Engineering Mathematics, E. Kreyszig John Wiley & Sons 10th Ed., 2015
2 Engineering Mathematics, N.P.Bali and Manish Goyal Laxmi Publishers, 7th Ed., 2007

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