UPTU Previous Year Exam Papers
B Tech 8th Semester
Plant Cell Biotechnology 2006-07
Note : (1) Attempt all questions.
(2) All questions carry equal marks.
(3) Use figures and diagrams to explain your answer.
1. Attempt any two parts of the following :
(a) Draw a well leveled neat plant cell and explain the process of differentiation and re differentiation.
(b) Discuss the various modes of regeneration of plant in nature and in laboratory.
(c) Compare the nutrients’ requirement of plant tissue culture with microbial culture. Also explain the factors affecting shoot-bud differentiation.
2. Attempt any two parts of the following :
(a) Discuss the Mechanical and Enzymatic methods of isolation of single plant cells. Also mention the subculture considerations of suspension culture.
(b) Explain the techniques of developing the high yielding cell lines. How will you check the viability of cultured cells?
(c) Discuss the mechanism and pattern of accumulation of serpentine in batch culture of Catharanthus rogeus.
3. Attempt any two parts of the following :
(a) Examine the advantages of plant cell culture over intact plants for secondary metabolites’ production. Enlist ten plant species and their corresponding products.
(b) Discuss the technological barriers encountered in mass cultivation of plant cells. Suggest the measures to minimize these barriers.
(c) Discuss the methods of plant cells’ immobilization. Also mention the application of immobilized cells in Bioreactor.
4. Attempt any two parts of the following :
(a) Describe the role of growth regulators and their effect when used in varying proportions.
(b) What are the major stages of in-vitro clonal propagation? Why variation in phenotype of the plants is observed even if they are derived from the single cell?
(c) Discuss the configurations of different types of bioreactors used for large scale plant cell culture.
5. Attempt any two parts of the following :
(a) What is the mode of interaction in elicitor formation? Discuss the methodology of elicitation.
(b) Discuss the transformation of plant cells using Agrobacterium system. Also explain the Integration and Expression of foreign DNA in plant cells.
(c) Write in short on any two of the following :
(i) Chemical methods of synchronization of suspension culture
(ii) Ex-plant sterilization
(iii) Biotransformation using plant cell culture.