UPTU Previous Exam Papers
B Tech 4th Semester Enzyme Technology 2007
Note : (1) Attempt all questions.
(2) All questions carry equal marks.
1. Attempt any four of the following :
(a) Elaborate the following :
EC 2.3………………. ; EC 3.4….. ;
(b) Discuss the localization of Enzymes inside the plesmalma or mitochondrion.
(c) Differentiate between multifunctional enzyme and multienzyme complex.
(d) Define Isozynes. Name any two Isozymes.
(e) Define International Unit of Enzyme. How the enzyme activity of any Enzyme can be estimated ?
2. Attempt any two of the following :
(a) Discuss the effect of temperature on enzyme activity. How the Arhenius activation and deactivation energy can be determined?
(b) Discuss different types of competitive inhibition. Derive a rate equation for competitive inhibition enzyme system and how does it differ from non competitive one?
(c) Discuss the behaviour of Line-Weaver Burk plot for substrate inhibitory enzymatic reaction.
3. Attempt any two of the following :
(a) Discuss the mechanism and action of chymotrypsis.
(b) What is action of glyceraldehyde phosphate dehydrogenase. What are essential requirement for its action ?
(c) Discuss the action of aldolase and carboxypeptidase.
4. Attempt any two of the following :
(a) Discuss the role of ‘Protein engineering’ for structure and function of enzyme.
(b) Discuss the physico-chemical characterization of enzyme.
(c) Write a note on Protein Secondary Structure.
5. Attempt any two questions only :
(a) How the intracellular enzyme can be extracted from the cells ?
(b) What are the techniques available for determination of molecular weight of enzyme ? Discuss only one method in detail.
(c) Discuss various steps involved in the purification of enzymes.