NIT Syllabus Raipur 7th Semester Biotechnology Engineering

NIT-RAIPUR

VII SEM BIOTECHNOLOGY SYLLABUS

 

Name of the Subject Genomics Subject Code BT 20711 BT

Semester 7th Board of Studies Biotechnology

Maximum Marks 70 Minimum Marks 25

 

Unit 1: Introduction

 

Features of prokaryotic, eukaryotic & organellar genomes, Genome sizes- C value paradox,

Gene counting, Structural, Functional genomics. DNA sequencing: Principles of DNA

sequencing, Automated DNA sequencing, Shotgun sequencing- contig assembly.

 

Unit 2: Analysis of sequence data

 

Analysis & Annotation- ORF, Exon- Intron boundaries, Other features of nucleic acid

sequencing, Protein motifs & domains, Databanks, Sequence comparisons. Nature of genetic

variation- SNP, Methods to study variation- RFLP, PCR, based methods, Genome- wide

comparitions.

 

Unit 3

 

: Comparative Genomics

 

Human Genome Project, Phylogenetic Trees, Arabidopsis genome and other genome projects,

Synteny, Genome evolution.

 

Unit 4: Analysis of Gene Expression

 

Functional Genomics- Analysis transcription- Northern blot, RNase protection assay, RT-PCR,

Primer extension analysis, SI- nuclease protection assay, Comparing transcrioptomessubtractive

hybridization, different display, SAGE, Reporter genes.

 

Unit 5: Application

 

Gene in health and disease, Genomic disorders and molecular medicines, pharmacogenomics ,

Gene banks, Legal status of gene bank.

 

Text/ Reference Books

 

1. Handbook of Comparative Genomics: Principle and Methodology by Cecilia Saccone,

Graziano Pesole. Wiley- LISS Publication(2003).

2. Comparative Genomics by Meldy S. Clark.Kluwer Academyic Publishers(2001)

3. Essentials of Genomics & Bioinformatics C. W.Sensen, Wiley (2003).

4. Discovery Genomics, Proteomics & Bioinfo, A.M. Cambell, C.S.H. Press, (2003)

5. Various research and review journals like nature Biotechnology, Current option, Trends

and Annual Reviews.

 

National Institute of Technology, Raipur (C.G.)

 

DEPARTMENT OF BIOTECHNOLOGY SYLLABUS

 

Name of the

 

Subject

 

Microbial

 

Technology

 

Subject Code BT 20712 BT

 

Semester 7

 

th Board of Studies Biotechnology

 

Maximum Marks 70 Minimum Marks 25

 

Lecture

 

Periods/Week

 

Tutorial

 

Periods/Week

 

Practical

 

Periods/Week

 

Credits

 

L+P

 

03 01 03 4+2

 

Unit 1: Introduction

 

Introduction to Microbial Technology; Basic idea on fermentation process; Submerged,

Stationary, Solid and Semisolid with their merits and demerits. Media for industrial

fermentation.

 

Unit 2: Basic Requirements

 

Equipment and accessories for industrial processes. Microbial growth kinetics.

Sterilization;Batch sterilization and Continuous sterilization. Media Sterilization. Filter

Sterilization.

 

Unit 3

 

: Microbial Production

 

Production of Organic acid, Solvent, Antibiotics, Polysaccharides, Enzymes, Vitamins,

Pigments, Wine, Dextran, Aroma.

 

Unit 4: Microbial Product Modification processes

 

Isolation of Industrially important micro-organisms. Preservation of industrial important

microorganism. Improvement of microorganism. The Improvement of industrial strains

by modifying properties other than the yield of product.

 

Unit 5: Industrial and Medicinal Applications

 

Application of enzymes in industrially analytical purpose and medical therapy.

Biodegradation of cellulose and lignin. Production of Biodiesel and Rapeseed methyl

esters. Microbial metabolic products; Primary metabolites and Secondary metabolites.

 

Text Books:

 

1. Principles of Fermentation Technology, second edition, by P.F.Stanbery, A.

Witaker and S.J.Hall.

2. Manual of Industrial Microbiology and Biotechnology, second edition by L.

Demain and Julian E. Devies.

.

 

Reference Books:

 

1. Biotechnology by Dr. U. Satyanarayana

 

National Institute of Technology, Raipur (C.G.)

 

2. Industrial Microbiology by A. H. Patel.

 

National Institute of Technology, Raipur (C.G.)

 

DEPARTMENT OF BIOTECHNOLOGY SYLLABUS

 

Name of the

 

Subject

 

Unit Operations Subject Code BT 20713 BT

 

Semester 7

 

th Board of Studies Biotechnology

 

Maximum Marks 70 Minimum Marks 25

 

Lecture

 

Periods/Week

 

Tutorial

 

Periods/Week

 

Practical

 

Periods/Week

 

Credits

 

L+P

 

03 01 03 4+2

 

Unit 1

 

: Introduction

 

Solids, Characteristics of solid particles, types of standard screen series . Screening and

other separation methods: screen analysis, estimation of particle size, surface area and

particle population based on screen analysis, Storage and conveying of solids, ideal and

actual screens, principles of elutriation, flotation, jigging, cyclone separator, hydroclones,

electrostatics and magnetic separation processes.

 

Unit 2

 

: Size Reduction

 

Size reduction and enlargements, crushers, grinders, ultrafine grinders, energy and power

requirements in comminution, Crushing laws and work index, Open-circuit and closed

circuit operation.

 

Unit 3

 

:- Mixing and Agitation

 

Axial and radial flow impellers, prevention of vortex and swirling, Liquid-Liquid,

Liquid-Solid, Solid-solid mixing operations and equipments, power consumption in

agitated vessels mixing index.

 

Unit 4

 

: Sepration Techniques

 

Sedimentation, settling velocity, flocculation, thickener, thickener design, Classifier.

Filtration, filter media, filter aids, batch and continuous filtration, filtration equipments,

filter press, leaf, cartridge, vacuum filter, rotary drum filters. Advanced separation

processes: Dialysis, ultrafiltration, reverse osmosis and membrane separation.

 

Unit 5

 

: Conveyors

 

Conveyors, Belt conveyor, bucket elevator, flight conveyor, apron conveyor, screw

conveyor, pneumatic conveying.

 

Name of text books

 

:

1. Unit operations of Chemical engineering by W.L. McCabe

2. Unit operations I by Hiramath Kulkarni

3. Unit operations I by K. A. Gavhane

 

National Institute of Technology, Raipur (C.G.)

 

DEPARTMENT OF BIOTECHNOLOGY SYLLABUS

 

Name of the Subject Bioprocess

 

Technology

 

Subject Code BT 20731BT

 

Semester 7

 

th Board of Studies Biotechnology

 

Maximum Marks 70 Minimum Marks 25

 

Lecture

 

Periods/Week

 

Tutorial

 

Periods/Week

 

Practical

 

Periods/Week

 

Credits

 

L+P

 

04 01 00 5

 

Unit 1

 

Food Industry: Introduction to food industry, food storage, food processing, consumer

food processing, dairy products, fruits and vegetable products, poultry and meat product

 

Unit 2- Biotechnology:

 

Genetic engineering, Introduction to biotechnology, Pharmaceutical

industries, Agriculture application, petroleum products and role of biotechnology in field of

pollution control

 

Unit 3

 

Pharmaceutical Industry & Polymer Industry: Growth of industry, Economy of

industry, methods of production of penicillin Polymerization: Chemistry of polymerization

Engineering properties of polymers. Technology: Plastic, rubber, polymer oils, fibers

 

Unit 4

 

Paper & Pulp Industry : Paper and Pulp industry, Agents, principles, equipments,

technology

 

Unit 5

 

Oil & Wax Industry, Soap & Detergent Industry: Technology of oil, fats and waxes,

soap and detergents industries

 

Name of Text Books:

 

1. Groggings, Unit Processes in Organic Synthesis

2. Shreve, Chemical Process Industries.

 

Name of Reference Books:

 

1. Dryden, Outlines of Chemical Technology.

 

National Institute of Technology, Raipur (C.G.)

 

DEPARTMENT OF BIOTECHNOLOGY SYLLABUS

 

Name of the Subject Cancer Biology S u b j e c t C o d e BT 20732 BT

 

Semester 7

 

th Board of Studies Biotechnology

 

Maximum Marks 70 Minimum Marks 25

 

Lecture

 

Periods/Week

 

Tutorial

 

Periods/Week

 

Practical

 

Periods/Week

 

Credits

 

L+P

 

04 01 00 5

 

Unit 1

 

: Introduction

 

Introduction to Cancer Biology, phenotypic characteristic of cancer cell. Cell cycle and

proliferation- cyclins, cdk’s and detection of tumour cell growth. Cell cycle checkpoints.

 

Unit2

 

: Oncogenesis

 

Oncogenes and tumour suppressor genes.Genetic Basis of cancer– mutational incident.

Cytogenesis abnormalities, BRCA genes. Introduction to retroviruses.

 

Unit 3: Apoptosis

 

Apoptosis and cancer– overview of cell death process, apoptosis signaling pathways,

abnormalities and detection. Types of cancer.

 

Unit 4

 

: Metastasis

 

Tumour progression and metastasis– steps of metastasis, associated genes and organ specificity.

 

Unit 5

 

: Diagnosis and Therapy

 

Cancer therapeutics: surgical, chemotherapy, radiation and drug resistance. Cancer prevention–

chemoprevention,, hormone therapy and antioxidant treatment.

 

Name of Text Books:

 

1

 

. A. L. Lehringer, D.L. Nelson, M.M. Cox- “ Principles of werth publishers, 2000. 2. L. stryer,

J.M. Berge, J.L. Tymoezko-“Biochemistry “ W.H. freeman & Co. 2002.

 

Name of Reference Books:

 

1.

 

Cell & Molecular Biology “concepts & Experiments” Geratd Karp, John Wiley & Son’s. 2.

The Cell- A molecular approach, Gn Cooper Asm Press.

 

National Institute of Technology, Raipur (C.G.)

 

DEPARTMENT OF BIOTECHNOLOGY SYLLABUS

 

Name of the Subject Molecular

 

Pathogenesis

 

Subject Code BT 20733BT

 

Semester 7

 

th Board of Studies Biotechnology

 

Maximum Marks 70 Minimum Marks 25

 

Lecture

 

Periods/Week

 

Tutorial

 

Periods/Week

 

Practical

 

Periods/Week

 

Credits

 

L+P

 

04 01 00 5

 

Unit 1

 

General clinical laboratory techniques and procedures: Volumetric analysis,

balancing and weighing, concept of solute and solvent, units of measurement. Specimen

collection and processing from blood, urine, spinal fluid, saliva and synovial fluid. Sample

preservation and transportation.

 

Unit 2

 

Clinical Enzymology : Principle of diagnostic enzymology, liver, cardiac and skeleton

enzymes, digestive enzymes, etc.

 

Unit 3

 

General Function Test: Liver function test, cardiac function test, renal function test,

thyroid function test. Reproductive endocrine function test.

 

Unit 4

 

Immunodiagnostic: Immuno assay classification and commercial technologies. DNA

based diagnostic – PCR, RFLP, ASSCP, Micro array, and FISH. Cell based diagnostic-

Antibody markers, CD markers and bioassays.

 

Unit 5

 

Biosensors: Concept and applications, non invasive biosensors in clinical analysis.

Introduction to biochips and their applications in modern science.

 

Name of Text Books:

 

1. Commercial Biosensors.:- Graham Ramsay, John Wilay Son, INC. (1998).

2. Essentials of Diagnostic Microbiology . Lisa Anne shimeld.

 

Name of Reference Books:

 

1

 

. Diagnostic Microbiology Balley & Scott’s.

2. The Science of laboratory Diagnosis, Crocker Burnelt

 

National Institute of Technology, Raipur (C.G.)

 

Subject : Microbial Technology

 

Subject Code : BT20721BT

 

End Semester Exam Marks : 20

 

List of Experiments:

 

1. Microbial strain improvement and preservation techniques.

2. Amino acid production and purification.

3. Alcohol production.

4. Citric acid production.

5. Antibiotic production and screening.

6. Microbial degradation of soil pollutant.

7. Amylase production and analysis.

8. Screening and selection of auxotropic mutants.

 

List of Equipments/Machine Required:

 

1. Autoclave

2. Hot Air Oven

3. Laminar Air Flow

4. Microscope

5. Water Bath

6. Colony Counter

7. Digital Balance

8. Rotating Incubator

9. BOD Incubator

10. Distillation Unit

 

Text Books:

 

1. Principles of fermentation Technology, second edition, by Stanberry, Whitaker and Hall.

2. Industrial Microbiology by A.H. Patel

3. Experimental Microbiology, Plant Pathology and Biotechnology by K. R. Aneja

 

National Institute of Technology, Raipur (C.G.)

 

Subject: Unit Operation Lab

 

Subject Code: BT20722BT

 

End Semester Exam Marks : 20

 

List of Experiments :

 

1. To determine the size distribution of a sample of particulate solid by sieve analysis and

to evaluate the average particle diameter.

2. To determine the size distribution of the product of laboratory rod mill.

3. To determine the size distribution of the product of laboratory ball mill.

4. To evaluate the overall effectiveness of given screen.

5. To determine the power required in size reduction and to evaluate the Rittinger’s

Constant in respect of Laboratory Rod Mill.

6. To determine the degree of mixing of a given binary solid system in Tumbler Mixer.

7. To determine the size distribution in a mass of fine solids by the method of

decantation.

8. To study the settling characteristics of the given slurry.

9. To determine the power required for crushing in Roll Crusher.

10. Study of separation of two liquids in laboratory Centrifuge.

11. Study of Filter Press.

12. To determine the power required in size reduction and to evaluate the Rittinger’s

Constant in respect of Ball Mill.

 

List of Equipments/Machine Required:

 

1. Sieve Shaker

2. Rod mill

3. Ball mill.

4. Tumbler Mixer

5. Roll Crusher

6. Filter Press

7. Centrifuge

 

Recommended Books:

 

1. W. L. McCabe, J. C. Smith & Peter Harriott, ‘Unit Operations of Chem. Engg.’ 5

Th Ed. McGraw Hill Pub.

2. Badger & Banchero, ‘Introduction to Chemical Engg.’ McGraw Hill

3. Brown et al., ‘Unit operations’, John Wiley sons.

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