NIT Raipur Syllabus 5th Semester Biotechnology Engineering




Name of the Subject Animal Biotechnology

Subject Code BT 20511 BT

Semester 5th Board of Studies Biotechnology

Maximum Marks 70 Minimum Marks 25


Unit I – Introduction to Animal Tissue Culture


: Historical background, Advantages,

Limitations and Applications, Laboratory Design and Layout, Essential Equipments, General

Safety Measures, Aseptic Techniques: Objectives and Elements, Sterile Handling, Risk



Unit II – Media:


Culture environment, physicochemical properties, Types of Culture Media,

Balanced salts solutions, Complete Media, Serum, Disadvantages of Serum, Advantages &

Disadvantages of Serum-free media, Cell Proliferation and Differentiation.


Unit III – Primary and Secondary culture


: Isolation of Cells and Tissues, Steps involved in

Primary Cell Culture, Subculture and propagation, Concept of Cell Lines and Nomenclature,

Cell Line Designations, Routine maintenance, Cell Immobilization.


Unit IV- Characterization & Quantization of Cell Lines


: Morphological studies:

Chromosome analysis, DNA content, RNA and Protein, Enzyme Activity, Antigenic Markers.

Transformation: Immortalization, Aberrant Growth, Tumorigenicity. Transformation Assay, Cell

counting, Cell Proliferation and Viability assay.


Unit V – Animal Biotechnology


: Animal Cloning, Transgenic Animal Production:

Microinjection method, Retroviral vector method and Other Techniques, Applications of

transgenic animals. Embryonic Stem Cell Technology,

In-vitro Fertilization and Embryo

Transfer Technology, its Significance.


Text/ Reference Books


1. Animal Cell Culture by John R.W. Masters, Oxford University Press.

2. Molecular Biotechnology: Primrose

3. Animal cell Biotechnology and: R.E.Spier and J.B. Griffiths(1988), Academic press.

4. Introduction to cell & tissue culture by Jennie P. Mather and Penelope E. Roberts Plenum

Press, New York and London.


National Institute of Technology, Raipur (C.G.)




Name of the Subject Bioethics &




Subject Code BT 20514 BT


Semester 5


th Board of Studies Biotechnology


Maximum Marks 70 Minimum Marks 25


















03 01 00 04


Unit I – Introduction to Ethics & Bioethics


Ethical issues in genetic engineering, patenting human

genes, cloning, genetic testing & screening; Biotechnology & social responsibility; The

legal & socio-economic impact of Biotechnology; Public acceptance issue in

Biotechnology-issue of access, ownership, monopoly, traditional knowledge, public

verses private funding.


Unit II – Intellectual property Rights


TRIPS; GATT; International Conventions; Patent-basic

principle & requirements, Patent application, Patentable subject & patenting living

organisms; Biodiversity& Farmer’s rights;


Unit III – Biosafety regulatory frame work


for GMOs in India; Biosafety regulatory frame work

for GMOs at International level.


Unit IV – Hazard assessment


Use of genetically modified organisms & their release in environment;

special procedures for rDNA based product production; Biosafety in laboratory-

Laboratory associated infections and other hazards; assessment of biological hazard &

level of Biosafety; Prudent biosafety practices in laboratory.


Unit V- International dimensions


in Biosafety- Cartagena Protocol on biosafety; Bioterrorism &

conventions on biological weapons.


Name of Text & Reference Books:


1. Thomas J.A., Fush R.L., (2002), Biotechnology & safety Assessment (3

rdEd.), Academic


2. Fleming D.A., Hunt D.L., (2002), Biological safety Principles & practices( 3

rd Ed.) ASM

Press, Washington.

3. Biotechnology- A Comprehensive treatise (Vol 12), Legal economic & ethical

Dimensions VCH.

4. Sasson A, Biotechnologies & Development, UNESCO Publications.

5. Singh K, Intellectual Property Rights on Biotechnology, BCIL, New Delhi.


National Institute of Technology, Raipur (C.G.)




Name of the




Drug Design and


Pharma Tech.


Subject Code BT 20513 BT


Semester 5


th Board of Studies Biotechnology


Maximum Marks 70 Minimum Marks 25


















03 01 00 04


Unit 1: Introduction and historical perspective


: Biotechnology: An introduction,

Pharmacist and Biotechnology, Biotechnology and Industry, GMP Compliance and

Biopharmaceutical facilities.


Unit 2: Biopharmaceutics:


Introduction, routes of administration. Pharmacokinetics;

Absorption of drugs, distribution of drugs, protein binding of drugs, drug metabolism and

Excretion of drugs.


Unit 3: Drug Delivery:


Molecular principle of drug Targeting and Liposomes. Delivery

consideration of Biotechnological products: Stability profile, Barriers to peptides and

protein delivery, Delivery of protein and peptide drugs, Site specific protein modification,

Toxicity profile characterization.


Unit 4: Recombinant DNA Technology:


Application of Recombinant DNA technology:

Novel protein generation, Novel routes to small molecules, Cloning of hemoglobin in Ecoli,

Cloning of human artificial receptors for drug design and testing. Drug delivery

system in Gene Therapy.


Unit 5: Current Trends in Vaccines:


vaccine; Multivalent subunit vaccine, Purified

macromolecules, Synthetic peptide vaccines, Recombinant antigen vaccine, Vector

vaccines, Miscellaneous approaches, New generation vaccines, Novel vaccine delivery



Text Books:


1. A Text book of Pharmaceutical Medicine (fourth edition) Edited by John P

Griffin and John O Grady.

2. Pharmaceutical microbiology – Hugo Russel’s Black well Publication 7

th Edition.


National Institute of Technology, Raipur (C.G.)


Reference Books:


1. Pharmaceutical Biotechnology S. P. Vyas, V.K.Dixit , CBS Publishers and


2. Essential of Pharmacotherapeutics F.S.K. Barar, S.Chand and company Ltd.


National Institute of Technology, Raipur (C.G.)




Name of the








Subject Code BT 20512 BT


Semester 5


th Board of Studies Biotechnology


Maximum Marks 70 Minimum Marks 25


















03 01 02 4+2


Unit 1: Introduction of Enzyme:


Brief history, nomenclature & classification,

Coenzyme and Cofactors, Induced & Lock-Key hypothesis, Transition-state stabilization

hypothesis, Zymogen, Ribozyme & Abzymes, Allosteric enzymes.


Unit 2: Enzyme specificity and activity:


Types of specificity, Identification of binding

site & catalytic site, 3-D structure of active site, Kinetics of single and bi-substrate

enzyme catalysed reactions, Inhibition & its kinetics, factors affecting enzyme activity.


Unit 3: Enzyme immobilization:


Methods of immobilization of enzymes-physical &

chemical techniques, Kinetics of immobilized enzyme, Effect of external mass transfer &

intra-particle diffusion, limitation & applications of immobilized enzymes, Bioreactors

using immobilized enzyme.


Unit 4: Production and Application:


Methods of production of enzyme, Extraction of

enzyme of soluble and membrane bound enzymes, Nature of extraction medium,

purification of enzyme, criteria of purity, determination of molecular weight of enzyme.

Application of enzyme in leather industry, detergent industry, dairy industry;

Lignocellulose degrading enzymes.


Unit 5: Enzyme Engineering:


Introduction, aim, principle & steps of enzyme

engineering; Prediction of enzyme structure, design and construction of novel enzymes,

Bifunctional and polyfunctional enzyme, Enzyme in organic solvents.


Text Books



1. Enzyme by Palmer (2001); Horwood publishing series.

2. Fundamental of Enzymology by Price and Stevens (2002): Oxford University



Reference Books



1. Enzyme technology by Helmut uhling (1998): John Wiley

2. A. L. Lehringer, d.L. Nelson, M.M Cox- “Principle of Biochemistry “ by Werth

publishers, 2000.

3. L. Stryer, J.M. Berge, J.L. Tymoezko-“Biochemistry” W.H. freeman & Co. 2002

4. Introduction to protein structure by B randen and Tooze(1998): Garland

publishing group.


National Institute of Technology, Raipur (C.G.)


National Institute of Technology, Raipur (C.G.)




Name of the




Fluid Flow




Subject Code BT 20516 BT


Semester 5


th Board of Studies Biotechnology


Maximum Marks 70 Minimum Marks 25


















04 01 00 05


Unit 1: Introduction :


Properties and nature of fluids – ideal fluid – real fluid – density –

specific weight – specific volume, surface tension, compressibility, capillarity – absolute

and gauge pressures.Fluid statics – forces on fluids, hydrostatic equilibrium –

measurement of pressure using manometer – U-tube manometer, differential manometer,

inverted manometer, micromanometer, manometers connected in series.Continuous

gravity decanter – Centrifugal decanter. Forces on submerged bodies – Buoyancy –

stability of floating and submerged bodies.


Unit 2: Introduction to fluid flow


: Flow of incompressible fluid – classification of flow

– steady and unsteady state flow, uniform and non-uniform flow – Three, two and onedirectional

flow – streamline, streak line, path line, stream tube, velocity potential,

laminar flow.


Unit 3


: Equations of change for isothermal systems


– equation of continuity, equation of motion – Navier-Stoke’s equation, Euler equation –

Newtonian and non-Newtonian fluids, viscosity, momentum flux, Reynold’s experiment,

turbulent flow, turbulence, nature of turbulence, flow in boundary layers – boundary layer

separation, wake formation- flow through pipe line system – Bernouilli equation’ kinetic

energy correction factors – correction in Bernouilli equation for fluid friction – Shear

stress and velocity distribution in circular channel. The friction factor – Hagen-Poiseuille

equation, laminar flow of non-Newtonian fluids – Velocity distribution for turbulent flow.

The friction factor chart – commercial pipes and pipe fittings. Friction head loss for

changes in velocity, direction and due to pipe fittings.


Unit 4: Transportation and metering of fluids


– pipes and pipe standards, tubings.

Pipe joints – flange – expansion joints, valves, automatic control valves – material of

construction. Water hammer – Pumps, reciprocating pumps, centrifugal pumps,

centrifugal pump theory – selection of centrifugal pumps – various types, head Vs. flow

rate – characteristics of centrifugal pumps. The displacement and current meters –

variable area meter, orifice meter, venturimeter, flow nozzles, rotameter, wiers and

notches – Pitot tubes – velocity meters – anemometers, turbine flow meter, current meters,

hot wire anemometer, laser dopper anemometry, flow visualization, Microfluidics.


Text Book


1. McCabe and Smith, “Unit Operations in Chemical Engineering”, McGraw-Hill


National Institute of Technology, Raipur (C.G.)


2. Streeter, “Fluid Mechanics”


Reference Books:


1. Christie J. Geankoplis, “Transport Processes and Unit Operations”, Prentice Hall of



National Institute of Technology, Raipur (C.G.)




Name of the




Immunology Subject Code BT 20515 BT


Semester 5


th Board of Studies Biotechnology


Maximum Marks 70 Minimum Marks 25


















03 01 02 4+2


Unit 1: Overview of Immune System:


Innate and Adaptive Immunity, Cells and Organs

of the Immune System, Hematopoiesis, MHC.


Unit 2: Immunoglobulins and Antigens:


Structure, Function and Classification of

Immunoglobulins. Structure and function of Antigens, epitopes. Isotype, Allotype and

Idiotype. Cytokines and its regulation.


Unit 3


: Genetics of Immunoglobulins: Two gene model and verification, DNA

rearrangements, VDJ joining and its different mechanisms, Class Switching, Expression

of Ig genes and its Regulation Antibody genes and Antibody Engineering.


Unit 4: Immune Response and Defects:


Introduction of complement system, Basic

concepts of Ag-Ab interactions, Immunodeficiency disorders, AIDS and SCID.

Autoimmunity Diseases.


Unit 5: Transplantation and Vaccines :


Graft Acceptance and Rejection and its

Mechanism, Tumor Immunology, Vaccines, Hypersensitivity.


Name of Text books:


1. Kubey’s Immunology

2. Immunology by Ivan Roitts


National Institute of Technology, Raipur (C.G.)


Subject : Enzyme Technology Lab


Subject Code : BT20522BT


End Semester Exam Marks : 20


List of Experiments :


1. Production of Amylase by A. Niger.

2. Effect of temperature/pH/concentration on salivary amylase activity.

3. Production of catalase enzyme by microorganisms.

4. Production of lactase by yeast cell.

5. Various Techniques of enzyme immobilization.

6. Study of various enzyme reactors.

7. Various techniques for enzyme engineering.

8. Preparation of poly/bifunctional enzymes.

9. Test for urease activity.

10. Test for oxidase activity.

11. Test for coagulase activity.

12. Test for Gelatin Hydrolysis. (proteolytic Activity )

13. Indole production test

14. Methyl Red test

15. Citrate utilization test


National Institute of Technology, Raipur (C.G.)


Subject: Fluid Flow Operation Lab


Subject Code: BT 20521BT


End Semester Exam Marks: 20


List of Experiments:


1. To determine coefficient of discharge of Venturimeter and Orificemeter.

2. To determine the velocity coefficient of closed circuit Pitot tube apparatus.

3. To calibrate given Rotameter.

4. Verification of Bernoulli’s theorem.

5. To determine over all heat transfer coefficient of double pipe heat exchanges.

6. To determine over all heat transfer coefficient of shell and tube heat excluder.

7. Determination of coefficient of friction for different pipes.

8. To determine Reynolds number of flowing fluid and draw fiction factor curve.

9. To study the flow over a Triangular or Rectangular notch or weir and to find the value of

coefficient of discharge (C


10. To determine fluidiction coefficient of fluidized bed.

11. Study of humidifier.

12. Study of spray dryer

13. Determine the heat transfer coefficient for heat flow through composite wall.


List of Equipments/Machine Required:


1. Shell and tube heat exchanger

2. Sudden expansion and sudden contraction arrangement

3. Fluidized bed.

4. Spray dryer.

5. Pitot tube

6. Humidifier

7. Venturimeter and Orificemeter

8. Rotameter


Recommended Books:


1. McCabe and Smith, Unit Operation of Chemical Engg

2. Coulson and Richardson, Chemical Engineering, vol 1.

3. Treyball, Mass Transfer Operation


National Institute of Technology, Raipur (C.G.)


Subject: Immunology Lab


Subject Code: BT20515BT


End Semester Exam Marks: 20


List of Experiments :


1. Determination of Blood groups.

2. Estimation of Blood Hemoglobin.

3. Differential Count of Blood leucocytes.

4. Widal Test.

5. Antigen–Antibody precipitation test.

6. Sensitivity of Microorganisms to antibiotics

7. Determination of concentration of penicillin G using agar diffusion (cup plate) method

8. Minimum inhibitory concentration (MIC) of penicillin G.

9. Study of Blood Clotting mechanism.

10. Rh Factor Determination.

11. Slide Agglutination test for serotyping pathogens.

12. VDRL test for syphilis by antigen – antibody reaction.

13. ELISA.

14. Separation of Blood proteins by electrophoresis technique.


List of Equipments/Machine Required:


1. Haemocytometer

2. Incubator

3. Refrigerator

4. Microscope

5. Micro-pipette


Recommended Books:


1. Refer Books mentioned in theory syllabus

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