Anna University Exam Paper Metabolic Engineering

Anna University Exam Paper Metabolic Engineering

METABOLIC ENGINEERING

 

Max. Marks: 100                                                                                           Time: 3 hours

PART – A (10 X 2 = 20 Marks)

Answer ALL the Questions

1. What is MCA? Define flux control coefficient?

2. What is reverse isotope trapping and overloading technique? What is their use?

3. What is energy charge? Why is it important?

4. How are isocitrate dehydragenase and citrate synthase regulated?

5. What are the metabolic effects of glucagon on glucose metabolism?

6. With the help of only a diagram illustrate the various pathways for glucose –6- phosphate.

7. What are analog resistant mutants and revertants?

8. What is inverse metabolic engineering?

9. Define network rigidity, flexible and rigid modes.

10. What are the different means by which metabolic control is executed?

 

PART – B (4 X 20 = 80 Marks)

1) Answer any FOUR Questions.

2) Question No. 11 is Compulsory

11. What are the criteria to be employed in the choice of an organism for metabolite overproduction? List the sequence of steps and the strategy involved in

(a)    the isolation of a mutant of C.glutamicum overproducing lysine that does not recognize inhibitors or repressors.

(b)   The isolation of a mutant of C.glutamicum overproducing glutamic acid.

12. Describe the different techniques employed in the isolation of auxotrophs and ARM. Diagram a flow chart for the strain improvement programme for secondary metabolic production.

OR

What are the potentials and perils of rational design in the metabolic engineering cycle? How does evolutionary engineering fill gaps in the rational design cycle?

13. Give a blueprint for the selection of induced mutants synthesizing improved levels of primary metabolites with suitable examples of pathways.

OR

      Give a concise review of your seminar paper.

14. Give the central metabolic pathway in E.coli. Using techniques of genetic engineering and the strategies of metabolic engineering, describe how you will direct the flux of this pathway to overproduce propionic acid. How may one use evolutionary engineering towards this end?

15. Outline a strategy for the selection of induced mutants synthesizing improved levels of primary metabolites. How is it different from the methods involving secondary metabolic production?

 

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