AIIMS Syllabus for Biochemistry Pratical

AIIMS Biochemistry Practical Syllabus


1. Laboratory Instrumentation.
2. Protein fractionation, denaturation, separation of proteins and amino acids.
3. Colour reactions of amino acids and proteins.
4. Estimation of blood analytes: glucose, total cholesterol and HDL cholesterol, uric acid, electrolytes,
5. Cerebrospinal fluid analyses.
6. Gastric juice analyses.
7. Urine analyses.
8. Amniotic fluid analyses.
9. Enzymes: amylase, lactate dehydrogenase and alkaline phosphatase
10. Liver function tests
11. Renal function tests.
12. Gel electrophoresis of DNA.
13. Immunodiffusion techniques, RIA and ELISA
14. Case-oriented discussions (enzymes, metabolites, function tests)
Biochemistry is taught in two semesters in the 1st year of the MBBS curriculum.
1st Semester : Aug-December
2nd Semester : Jan-June
1. Didatic lectures: three and a half hours/week of interactive classroom lectures to facilitate learning
  of terminology, principles and concepts. Books and resource material are suggested to encourage
  self- directed learning.
2. Tutorials; Two hours/week. Problem based small group discussions, questions-answer sessions,
  revision and reinforcement of difficult concepts in tutorial hours. The purpose is to inculcate skills
  of reasoning, meaningful approaches to learning and facilitate understanding of the subject.
3. Laboratory exercises: (Biochemistry practicals) three hours/week: a) to substantiate and clarify
  theoretical concepts with experimental evidence b) to develop skills of performing basic biochemical
  tests important in clinical investigations c) to develope familiarity with biochemical laboratory
  instrumentations techniques.
  (1) Practical bench work


(2)     Demonstration

(3)     Analyses and interpretation of data

(4)     Discussions on the practicals with the help of clinical and scientific problems


  1. Biochemistry Ed. Lubert Stryer. W.H. Freeman and Company, New York.
  2. Principles of Biochemistry. Ed. Lehinger, Nelson and Cox. CBS Publishers and distributors.
    1. Harper”s Biochemistry, Ed. R.K. Murray, D.K. Granner, P. A. Mayes and V.W. Rodwell. Appleton and Lange, Stamford, Connecticut.
  3. Textbook of Biochemistry with Clinical Correlations. Ed. Thomas M. Devlin, Wiley-Liss Publishers.
  4. Tietz Textbook of Clinical Chemistry. Ed. Burtis and Ashwood. W.B. Saunders Company.
  5. Biochemistry. Ed. Donald Voet and Judith G. Voet. John Wiley & Sons, Inc.
  6. Immunology Ed. 5th Richard A. Goldsby, Thomas J Kindt, Barbara A Osborne, Janis Kuby.
  7. Immunology Ed. 6th Ivan Roitt, Jonathan Brostoff, David Male.


Semester examinations

1st mid Semester: held on 1st – 2nd week of October 1st end Semester: held on 1st – 2nd week of December Second Semester: held on 1st – 2nd week of March.

Semester examinations are held both for the theory and practical courses.

1st professional Final (Summative) Examination: Students are assessed on the complete 1st year syllabus in biochemistry theory and practicals.

Mark distribution

Semester examinations

Theory: total 75 (25 in each semester):

Practical: total 75 (25 in each semester)

Final Examination

Theory : Total 75

Practical : Total 75

Total marks (1st Prof. in Biochemistry)

Semester Examination 150 Final Examination    150

Total            300


Distribution of Topics

Paper I Cell structure, compartmentation, functions, biological membranes. Chemistry, functions and metabolism of amino acids, carbohydrates and lipids, metabolic interrelationships, in born errors of metabolism, nutrients and malnutrition, biological oxidation, hormones, vitamins and coenzymes.

Paper II Protein structure and functions, hemoglobin, myoglobin, plasma proteins and iron metabolism, enzymes and enzyme kinetics, nucleic acid biochemistry and molecular biology, immunology, digestion and absorption, function Tests: G.I.T., kidney, and liver, regulation of acid base balance, environmental biochemistry, cancer biochemistry.

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