AIIMS Biochemistry Practical Syllabus
|2.||Protein fractionation, denaturation, separation of proteins and amino acids.|
|3.||Colour reactions of amino acids and proteins.|
|4.||Estimation of blood analytes: glucose, total cholesterol and HDL cholesterol, uric acid, electrolytes,|
|5.||Cerebrospinal fluid analyses.|
|6.||Gastric juice analyses.|
|8.||Amniotic fluid analyses.|
|9.||Enzymes: amylase, lactate dehydrogenase and alkaline phosphatase|
|10.||Liver function tests|
|11.||Renal function tests.|
|12.||Gel electrophoresis of DNA.|
|13.||Immunodiffusion techniques, RIA and ELISA|
|14.||Case-oriented discussions (enzymes, metabolites, function tests)|
|Biochemistry is taught in two semesters in the 1st year of the MBBS curriculum.|
|1st Semester : Aug-December|
|2nd Semester : Jan-June|
|1.||Didatic lectures: three and a half hours/week of interactive classroom lectures to facilitate learning|
|of terminology, principles and concepts. Books and resource material are suggested to encourage|
|self- directed learning.|
|2.||Tutorials; Two hours/week. Problem based small group discussions, questions-answer sessions,|
|revision and reinforcement of difficult concepts in tutorial hours. The purpose is to inculcate skills|
|of reasoning, meaningful approaches to learning and facilitate understanding of the subject.|
|3.||Laboratory exercises: (Biochemistry practicals) three hours/week: a) to substantiate and clarify|
|theoretical concepts with experimental evidence b) to develop skills of performing basic biochemical|
|tests important in clinical investigations c) to develope familiarity with biochemical laboratory|
|(1) Practical bench work|
(3) Analyses and interpretation of data
(4) Discussions on the practicals with the help of clinical and scientific problems
- Biochemistry Ed. Lubert Stryer. W.H. Freeman and Company, New York.
- Principles of Biochemistry. Ed. Lehinger, Nelson and Cox. CBS Publishers and distributors.
- Harper”s Biochemistry, Ed. R.K. Murray, D.K. Granner, P. A. Mayes and V.W. Rodwell. Appleton and Lange, Stamford, Connecticut.
- Textbook of Biochemistry with Clinical Correlations. Ed. Thomas M. Devlin, Wiley-Liss Publishers.
- Tietz Textbook of Clinical Chemistry. Ed. Burtis and Ashwood. W.B. Saunders Company.
- Biochemistry. Ed. Donald Voet and Judith G. Voet. John Wiley & Sons, Inc.
- Immunology Ed. 5th Richard A. Goldsby, Thomas J Kindt, Barbara A Osborne, Janis Kuby.
- Immunology Ed. 6th Ivan Roitt, Jonathan Brostoff, David Male.
EXAMINATION & MARKS DISTRIBUTION
1st mid Semester: held on 1st – 2nd week of October 1st end Semester: held on 1st – 2nd week of December Second Semester: held on 1st – 2nd week of March.
Semester examinations are held both for the theory and practical courses.
1st professional Final (Summative) Examination: Students are assessed on the complete 1st year syllabus in biochemistry theory and practicals.
Theory: total 75 (25 in each semester):
Practical: total 75 (25 in each semester)
Theory : Total 75
Practical : Total 75
Total marks (1st Prof. in Biochemistry)
Semester Examination 150 Final Examination 150
Distribution of Topics
Paper I Cell structure, compartmentation, functions, biological membranes. Chemistry, functions and metabolism of amino acids, carbohydrates and lipids, metabolic interrelationships, in born errors of metabolism, nutrients and malnutrition, biological oxidation, hormones, vitamins and coenzymes.
Paper II Protein structure and functions, hemoglobin, myoglobin, plasma proteins and iron metabolism, enzymes and enzyme kinetics, nucleic acid biochemistry and molecular biology, immunology, digestion and absorption, function Tests: G.I.T., kidney, and liver, regulation of acid base balance, environmental biochemistry, cancer biochemistry.