AIIMS Microbiology Syllabus

AIIMS Microbiology Syllabus



  1. Microscopy and micrometry

–          Introduction to microscopes

–          Focussing slides under low/ high power and oil immersion

–          Principles and demonstration of various types of microscopes

  1. Direct demonstration of bacteria by staining

–          Gram staining

–          Albert’s staining

–          Acid fast staining

  1. Motility tests and biochemical tests for bacterial identification

–          Hanging drop method for motility testing

–          Important biochemical tests : principles and interpretation

  1. Laboratory diagnosis of viral infections

–          Collection and transport of samples

–          Demonstration of egg inoculation techniques, cell culture, cytopathic effect, plaque assay, serological tests (complement fixation, haemagglutination inhibition, neutralization, ELISA)

  1. Laboratory diagnosis of fungal infections

–          Collection and transport of specimens

–          Gram stain, KOH preparation, India ink preparation for direct demonstration

–          Sabouraud’s dextrose agar/media

–          Lactophenol cotton blue for identification

–          Latex agglutination test

  1. Sterilization and disinfection

–          Visit to media and sterilization room (demonstration of autoclave and hot air oven)

–          Visit to CSSD

  1. Stool examination for cysts

–          Collection and transport of stool sample for parasites

–          Direct examination (saline and iodine preparations)

–          Concentration of stool for parasites

–          Identification of cysts


Didactic lectures (DL) : 16 Intra-departmental seminars (IDS) : 3 Integrated seminars (IS) : 2


Practical sessions : 7                                                 14 hours

TOTAL : 40 hours (16 one hour sessions and 12 two hour sessions)

IV   SEMESTER Infections of gastrointestinal tract (contd.)





  1. Intestinal nematodes
  2. Intestinal nematodes

Intestinal cestodes and trematodes

  1. Intestinal cestodes
  2. Enterobacteriaceae
  3. Bacterial diarrhoea and dysentery

6       .        Cholera

  1. Cholera
  2. Food Borne Pathogens
  3. Bacterial food poisoning
  4. Helicobacter pylori
  5. Viral gastroenteritis

(Classification, epidemiology, life cycles etc.)

(Clinical features and lab diagnosis)

(Focus: only those seen in india; incl. cysticercosis)

(Epidemiology, clinical features, microbiological aspects, diagnosis)

(Introduction, common features, classification, infections caused – enumeration only)

(Focus: e.coli and shigella)

(Bacteriology, virulence, toxins, pathogenesis)

(Clinical Features, Epidemiology, lab diagnosis, vaccines)

(Aetiology, toxins, types, etc. include: introduction to staphylococcus and salmonella)

(PLUS: 1 I.S. should be taken by clinical department)

(To be accomodated by paediatrics involving microbiologists IN I.S.)

Infections of the G.I.T.

(Micro, Gastro)

(Micro, Gastro, Neuro)

(micro, gastro, paeds)

(Micro, Med,

Paeds, Comm. Med)

(Micro, Med, Paeds)


Infections of the respiratory tract




(Group A, B, B: bacteriology, pathogenesis, infections, lab diagnosis), and, introduction to viridans group and pneumococcus





  1. Sequelae of streptococcal infection
  2. Diphtheria
  3. Diphtheria, whooping cough and acute viral respiratory infections of childhood
  4. Agents causing pneumonia

(bacterial :incl. gnb, pneumococcus mycoplasma, chlamydia, legionella; viral; pneumocystis)




  1. Mycobacteria with special reference to Mycobacterium tuberculosis
  2. Infections due to Non- tubercular Mycobacteria (NTM) and Mycobacterium leprae
  3. Tuberculosis
  4. Tutorial: infections of respiratory tract

Urinary tract infection

  1. Urinary tract infections

Wound infections/anaerobic infections/fungal infections

  1. Staphylococcal infections – including bacteriology, pathogenesis

and lab diagnosis

  1. Anaerobic infections of clinical importance GNB anaerobes Clostridia
  2. Laboratory diagnosis of wound infections
  3. Dermatophytosis
  4. Mycetoma & subcutaneous mycosis
  5. Systemic mycoses Infections of the eyes
  6. Eye Infections
  7. Tutorial



  1. Stool examination for intestinal nematodes and cestodes

—      Collection/transport and concentration of sample

—      Identification of ova of intestinal nematodes and cestodes

—      Identification of adult worms and larvae

  1. Enterobacteriaceae

—      Common media and biochemical tests

—      Culture characteristics of members of Enterobacteriaceae

  1. Laboratory diagnosis of E.coli infection and shigellosis

—      Stool examination for pus cells and RBCs

—      Processing of stool specimen for bacterial culture

—      Cultural characteristics, tests for E.coli and its virulence factors

—      Cultural characteristics of Shigella and its identification (incl. slide agglutination test)

  1. Laboratory diagnosis of cholera

—      Collection and transport of specimen

—      Culture media and characteristics

—      Identification (incl. motility, oxidase and other tests)

—      Biotyping and serotyping

  1. Laboratory diagnosis of food poisoning

—      Focus on: laboratory diagnosis of salmonellosis

—      Demonstration for Clostridium perfringens and others

  1. Laboratory diagnosis of filariasis

—      direct examination/staining for microfilaria

—      demonstration of other aspects of filariasis – including vector

—      include revision of stool examination for helminthic ova

—      include demonstration of hydatid cyst

  1. Laboratory diagnosis of upper respiratory infections

—      Focus on: laboratory diagnosis of Streptococcus infection

—      Albert’s stain

—      Media, identification and toxin of Corynebacterium (demonstration)

  1. Laboratory diagnosis of lower respiratory tract infections

—      Focus on: Klebsiella and Streptococcus pneumoniae


—      Viral respiratory infection (demonstration of diagnostic methods)

  1. Laboratory diagnosis of tuberculosis

—      Collection of specimens (focus: sputum)

—      Concentration methods

—      Acid fast staining

—      LJ medium and culture characteristics

—      Differentiation between Mtb and NTM (basic concept/demonstration)

  1. Laboratory diagnosis of UTI

—      Collection, storage and transport of urine

—      Significant bacteriuria and quantitative/semiquantitative methods of culture

—      Media: including CLED

—      E.coli/ Klebsiella (revision)

—      Focus on: Identification of Proteus and Pseudomonas – cultural characteristics like swarming, pigment production; and tests like OF and oxidase

  1. Laboratory diagnosis of wound infections

—      Focus on: Staphylococcus (culture/ identification including tests like catalase and coagulase)

  1. Laboratory diagnosis of anaerobic infections

—      Demonstration of collection of samples for anaerobic culture

—      Methods of anaerobiasis – RCMB, anaerobic jar

—      Demonstration of media and culture for Clostridium, smears showing sporing and non-sporing GPB, Nagler’s reaction etc. and cultures of GN anaerobes like B.melaninogenicus

  1. Laboratory diagnosis of superficial, subcutaneous and deep mycoses


Didactic lectures (DL) : 17 Intra-departmental seminars (IDS) : 1 Integrated seminars (IS) : 13 Tutorials : 3 Practical sessions : 13

TOTAL : 74 hours (20 one hour sessions and 27 two hour sessions)


Pyrexia of Unknown origin AND OTHER FEBRILE ILLNESSES

  1. (1A. and 1B.) Enteric fever
  2. Malaria

2a. (Transmission, species, life cycle and stages)

2b. (Clinical features, lab diagnosis, prevention etc.)


  1. (3a. and 3b.) Leishmaniasis
  2. Childhood Viral ExAnthema
  3. Viral haemorrhagic fever
  4. Rickettsial infection with special reference to Indian rickettsial infection (Indian tick typhus, murine typhus, endemic typhus, scrub typhus
  5. Spirochetal infections other than Treponema pallidium (Borrelia, Leptospira)
  6. Septicaemia / Bacteraemia
  7. Tutorial: P.U.O.

Central Nervous system Infections

  1. Meningitis
  2. Encephalitis
  3. Poliomyelitis
  4. Rabies
  5. Cysticercosis and other CNS Parasitic diseases
  6. Slow viruses and prions
  7. Tutorial : CNS infections Sexually Transmitted Diseases
    1. Syphilis
    2. STD (incl. bacteriology of neisseria gonorrhoeae and other
    3. STD (clinical features and lab diagnosis)
    4. Herpes viruses Herpes viruses – Part 1 Herpes viruses – Part 2
    5. Human Immunodeficiency virus

Congenital infections

  1. Congenital infections
  2. Tutorial: Congenital infections and std Miscellaneous
    1. Hospital infection, universal precautions and waste management
    2. Zoonoses (Bacterial, viral, parasitic, fungal)
    3. Entomology of medical importance

(Micro, Dermatol, Med) (Micro, Paeds)

In clinical semesters (incl. Micro)

(Etiology, Lab diagnosis Pathogenesis) (Micro, Med, Paed, Neuro)

By Paeds / Community Medicine, (Incl. microbiology)

(Micro, Comm. Med.) by Neurology (INCL. microbiology)


(Micro, Derma, Obs & Gyn)

(Micro, Med, Paeds, Comm Med)

(Micro, Paeds, Obs & Gyn)

(Micro, surgery, paeds, ICU, Hosp Admn)



  1. Laboratory diagnosis of malaria

—      Preparation, staining and examination of peripheral blood smear for malarial parasite

—      Identification of various species and stages

—      Thick smear

—      Demonstration of vector and newer methods

  1. Laboratory diagnosis of leishmaniasis

—      Sample collection

—      Demonstration of LD bodies

—      Demonstration of vector, culture methods and serological tests

—      Revision of peripheral blood smear examination

  1. Laboratory diagnosis of enteric fever

—      Sample collection methods and transport

—      Blood culture (in detail)

—      Stool and urine culture for Salmonella

—      Widal test

—      Identification tests and slide agglutination for Salmonella

  1. Laboratory diagnosis of meningitis

—      Collection, aliquoting and transport of CSF

—      Other useful specimens

—      Direct smear examination

—      Culture media, growth characteristics and identification tests (focus: Neisseria, Haemophilus and Streptococcus pneumoniae)

—      Chronic meningitis: pathogens (demonstration e.g. India ink for Cryptococcus)

—      Antigen detection

  1. Laboratory diagnosis of STD

—      Collection and transport of specimens

—      Direct demonstration

—      Serological tests (focus: syphilis, HIV)

  1. Entomology

—      Demonstration of medically important entomological specimens


Didactic lectures (DL) : 12 Intra-departmental seminars (IDS) : 4

Integrated seminars (IS) : 10

Tutorials : 3 Practical sessions : 6

TOTAL : 55 hours (15 one hour sessions and 20 two hour sessions)


Teaching microbiology to undergraduate medical student is provided with the help of Didactic Lectures, Intradepartmental Seminars (IS), Integrated Seminars and Tutorials that deal with the etiology, pathogenesis, laboratory diagnosis, treatment and control of infections.


  1. Microscopy and micrometry
  2. Direct demonstration of bacteria by staining
  3. Motility tests and biochemical tests for bacterial identification
  4. Laboratory diagnosis of viral infections
  5. Laboratory diagnosis of fungal infections
  6. Sterilization and disinfection
  7. Stool examination for cysts, intestinal nematodes and cestodes
  8. Laboratory diagnosis of E.coli infection,shigellosis and cholera
  9. Laboratory diagnosis of food poisoning
  10. Laboratory diagnosis of filariasis
  11. Laboratory diagnosis of upper and lower respiratory tract infections
  12. Laboratory diagnosis of tuberculosis
  13. Laboratory diagnosis of UTI
  14. Laboratory diagnosis of wound infections
  15. Laboratory diagnosis of anaerobic infections
  16. Laboratory diagnosis of superficial, subcutaneous and deep mycoses
  17. Laboratory diagnosis of malaria
  18. Laboratory diagnosis of leishmaniasis
  19. Laboratory diagnosis of enteric fever
  20. Laboratory diagnosis of meningitis
  21. Laboratory diagnosis of STD
  22. Entomology : Demonstration of medically important entomological specimens



1. Medical Microbiology

  1. Jawetz, Melnick and Adelberg’s Medical Microbiology
  2. Parasitology ( Protozoology and Helminthology)

Greenwood Slack, Peutherer

Geo F. Brooks, Stephen A. Morse, Janet

S. Butel

K.D. Chatterjee











Five assessments in theory and practical are held as given below




III Semester

Mid Term





End Term




IV Semester

Mid Term





End Term




V Semester

End Term









Professional Examination


Paper I ( General Microbiology)


25 Marks


PaperII ( Systemic Microbiology)


25 Marks


Practical Examination


50 Marks



Theory Papers: Question paper is comprised of various types of MCQ (Single response, Multiple true-false, Matching, Reason Assertion, Multiple Completion) and short Answer Questions (SAQ).

Practical: Practical examination include spotting, different bacterial staining procedures, identification of bacterial strains using conventional methods.

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