An Introduction To Biotechnology: A Genetic Manipulation Perspective

By Rup Lal more
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Product Specifications

Publisher IK International All Biotechnology books by IK International
ISBN 9789384588861
Author: Rup Lal
Number of Pages 216
Available
Available in all digital devices
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An Introduction To Biotechnology: A Genetic Manipulation Perspective - Page 1 An Introduction To Biotechnology: A Genetic Manipulation Perspective - Page 2 An Introduction To Biotechnology: A Genetic Manipulation Perspective - Page 3 An Introduction To Biotechnology: A Genetic Manipulation Perspective - Page 4 An Introduction To Biotechnology: A Genetic Manipulation Perspective - Page 5

An Introduction To Biotechnology: A Genetic Manipulation Perspective by Rup Lal
Book Summary:

An Introduction to Biotechnology: A Genetic Manipulation Perspective contains practical exercises, and has a comprehensive coverage on the subjects of biotechnology and gene manipulation for the students' understanding of the basic concepts. This manual contains, in particular, the techniques, protocols and practical approaches that are being used routinely in molecular biology laboratories.

This book is assembled together with an aim to enlighten the readers with the complex molecular biology protocols in a simple and straightforward manner. In addition, this manual also introduces readers to the study of gene manipulation in Gram-negative as well as Gram-positive bacteria. All these methods and protocols have been regularly used in the editor's laboratory and have been modified from time to time. This practical handbook overcomes the limitations of other manuals by providing an in-depth methodology in a systematic way, blending the underlying theoretical aspects, thus facilitating the readers in understanding the reason behind every step followed during the experimental work.

Audience of the Book :
This book Useful for Biotechnology students.
Table of Contents:

1. Preparation of Media and Chemicals

2. Growth and Maintenance of Culture

3. Isolation of Genomic DNA

4. Isolation of Plasmid DNA

5. Isolation of RNA

6. Visualization of Nucleic Acids and Proteins

7. Whole Cell Protein Isolation and SDS Page

8. Quantification of DNA

9. Primer Designing

10. Polymerase Chain Reaction (PCR)

11. Restriction Digestion

12. Purification of DNA from an Agarose Gel

13. Ligation

14. Transformation

15. Cloning and Cloning Vectors

16. DNA Blot Hybridization

17. DNA Sequencing

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