About this eBook
Preface Bioanalytical techniques are used for qualitative and quantitative studies of a wide variety of biological systems. Choice of the right analytical technique requires considerable knowledge, experience and insight regarding the range of available techniques as well as the biological system. The aim of this book is to introduce the student to a wide variety of bioanalytical techniques and provide sufficient information regarding the principles, applicability and limitations associated with each method in the hope that this knowledge will enable the student to understand why a specific technique is used for a particular application. This book attempts to fill a void between books that explain the applications in detail but do not provide adequate description of the underlying principles and books that cover the physical principles underlying analytical techniques in great detail, but do not cover the extensions that form the basis of the standard applications in biological contexts. This book provides only a minimal description of the experimental protocols this is because specific protocols are subject to change due to rapid advances in technology, and are best obtained from the relevant recent publications. In addition, there is substantial variation in the standard protocols for instrumental techniques in many cases it is best to obtain the relevant protocol from the manufacturer of the instrument. I have not attempted an encyclopedic coverage of all bioanalytical techniques instead I have chosen to cover those techniques that the student is most likely to encounter now, and in the near future. Some specialized techniques have been introduced, either to emphasize a particular principle, or because of their current or potential importance. I have not attempted to cover all topics at equal length. Topics such as flame emission spectrometry that are covered in great depth in standard analytical chemistry books have been covered briefly, or omitted. Similarly, topics such as enzyme assays and immunoassays, covered well by standard books dealing with biochemical and molecular biological methods have also been covered very briefly.
2 14 2012, 3 55 PM
Modern methods, such as biosensors, biomicrofluidics and nanobioanalytical techniques, that have not yet appeared in standard introductory textbooks, have been introduced in this book. Although many specialized textbooks deal with these topics, most of these books