Scientific Wrinting Techniques And Project Management In Biotechnology By Dr. Simmi Kharb

Scientific Wrinting Techniques And Project Management In Biotechnology By Dr. Simmi Kharb
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Scientific Wrinting Techniques And Project Management In Biotechnology By Dr. Simmi Kharb

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Publisher: Laxmi Publications
ISBN: 9788131805381
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PREFACE The book titled Scientific Writing Techniques and Project Management in Biotechnology has been designed to provide a comprehensive basic text for medical and biotechnology students to make them conversant with molecular biology techniques, bioinformatics, art of planning and writing scientific research, issues of ethics and funding that arise while doing a research. In the past decades, the power of genetic analysis has grown with the introduction of recombinant DNA technology and genomics. Acquiring DNA sequence has become a routine Deciphering genetic information encoded in DNA sequence requires knowledge of what to look for, algorithms capable of detecting interesting features in sequences and computers to perform such complex analysis, leading to emergence of the new field of bioinformatics. Molecular biology is driven to a great extent by economics and a future researcher biotechnologist needs to be conversant with patenting issues biotech project management and product life cycle and capital investment and marketing, leading to emergence of a specialized subject biotechnology entrepreneurship. The book has separate sections on survey of techniques in molecular biology research and development of projects management of projects and marketing of biotechnology inventions and intellectual property and patenting. Although every effort has been made to ensure the correctness of the text, the author would be grateful to hear from any reader who discovers possible errors and inadequacies. Constructive suggestions for improvement of the text would be welcomed with due acknowledgement. AUTHOR
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TECHNIQUES IN MOLECULAR BIOLOGY INTRODUCTION Amazing developments in biotechnology in late twentieth century include decoding of human genome, cloning, gene therapy, progress in stem cell research and RNA interference. Many earlier landmark discoveries that unveiled the mysteries of genetics and paved way for modern molecular biology. Beginning with Mendel s experiments with garden peas published in 1866, suggesting concept of genes as discrete units of heredity. Morgan in 1910 revealed chromosomes as units of heredity and Avery in 1944 confirmed through bacterial studies that DNA carried genetic information with Watson Crick s DNA double-helical model of DNA in 1953, Smith demonstrated that DNA can be cleaved by restriction enzymes. This facilitated the subsequent development of RECOMBINANT DNA TECHNOLOGIES. With construction of genetic map using restricting enzymes by Nathans, Southern Blot was invented in 1975 to detect specific DNA sequences. This led to development of DNA sequencing methodologies and first complete DNA sequence of bacteriophage was discovered. PCR Polymerase Chain Reaction was developed by Mullis and Coworkers in 1985. Then, simultaneous interrogation of gene transcripts by DNA microarrays became a reality in 1996. The human genome sequence was completed in 2003. These great discoveries shaped the modern genetics, molecular biology and biotechnology.
TECHNIQUES IN MOLECULAR BIOLOGY Almost all the experiments dealing with gene manipulations require pure forms of