Oswaal CBSE Laboratory Manual For Class 12 Biology

Oswaal CBSE Laboratory Manual For Class 12 Biology
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Oswaal CBSE Laboratory Manual For Class 12 Biology

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Publisher: Oswaal Books
ISBN: 9789351276227
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IntroductioN to Biology practicals
INTRODUCTION TO BIOLOGY PRACTICALS Chemicals Commonly Us in Laboratory ed I. Commonly Used Stains 1. Aceto-carmine One gram carmine powder is dissolved in 45 glacial acetic acid. Solution is boiled gently. Few drop of ferric acetate are poured into the solution after cooling. The stain is filtered and stored in dark bottle. The stain is commonly used to study chromosomes during cell division. 2. Basic Fuchsin Eight gram basic fuchsin is dissolved in 100 mL ethyl alcohol by stirring. Stain is left for three days for maturation. After this, stain is filtered and stored in a dark bottle. The stain is used for DNA and chromosomal studies. 3. Borax carmine Four gram borax power and 3 gram carmine powder are dissolved in 100 mL distilled water. After this, 100 mL ethyl alcohol is poured into the mixture. The stain is left for 3-5 days for maturation. After this, stain is filtered and is used for normal histological studies. 4. Crystal violet Fourteen gram crystal violet is dissolved in 100 mL ethyl alcohol and the stain is left for 2-3 days for ripening. After this, the stain is filtered and can be used for chromosomal studies, and staining of bacteria and protozoans. 5. Eosin One gram eosin powder is dissolved in 100 mL distilled water. The stain is used for staining cytoplasm. 6. Haemotoxylin Four gram haemotoxylin is dissolved in 25 mL ethyl alcohol. After this, 400 mL saturated solution of ammonium alum is added. Solution is exposed to light for 48 hours. After this, 100 mL methyl alcohol and 10 mL glycerine are added to the stain. The solution is well mixed and kept for 2 months for ripening. The stain is filtered and stored. The stain is used for staining histological studies. 7. Iodine stain Ten gram potassium iodide is dissolved in 100 mL distilled water. After this, 5 g iodine is added by stirring. The stain is used for staining flagella, cilia and nuclei of the cells. 8. Leishman s stain One hundred and fifty mg Leishman s stain powder is dissolved in 100 mL methyl alcohol. The stain is used for staining blood cells. In this, nuclei of WBC are stained blue, RBC are stained pink and platelets are stained purple. 9. Methylene blue Five hundred mg sodium bicarbonate is dissolved in 95 mL distilled water. After this, 900 mg methylene blue powder is added. The solution is boiled for one hour and then cooled, filtered and stored. The stain is used for blood staining. 10. Safranin One gram safranin powder is dissolved in 100 mL of 50 ethyl alcohol. The stain is used for staining lignified tissues and nucleus.
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Oswaal CBSE Laboratory Manual For Class 12 Biology
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Publisher Oswaal Books
ISBN 9789351276227