Plant Tissue Culture

Plant Tissue Culture
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Plant Tissue Culture

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Publisher: Agrobios Publications
ISBN: 9789380655000
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Preface
tissue culture
plantlet culture micropropagation in vitro
Author
Contents
1 PLANT TISSUE CULTURE AN OVERVIEW ..............................1 Why Growth Occurs ............................................................................................................. 3 Kinetics of Growth or Measurement and Pattern of Growth............................... 4 What Happens When Growth Occurs .......................................................................... 4 Power of Regeneration in Plants ..................................................................................... 5 Why Plants Regenerate ..................................................................................................... 6 Callus Culture........................................................................................................................... 7 Meristem Culture.................................................................................................................... 7 Organ Culture .......................................................................................................................... 7 Contrasts between Plant and Animal Cells.................................................................. 8
2 HISTORY OF PLANT TISSUE AND CELL CULTURE ................12 20th Century Development................................................................................................13 Knop and Prdifer developed synthetic media ..........................................................13 Callus Culture of Carrots ...................................................................................................14 F. C. Steward ...........................................................................................................................15 H. E. Street ...............................................................................................................................15 C. O. Miller................................................................................................................................15 F. Skoog.....................................................................................................................................16 Toshio Murashige.................................................................................................................16 Vasil and Hilderbrandt ......................................................................................................16
Tissue Culture Researches in India ...............................................................................22 Bioinformatics .......................................................................................................................25 Gene Banking, Conservation and Exchange in India ............................................25
3 GENERAL METHODOLOGY OF PLANT TISSUE CULTURE ..........................................................................27 Cabinets or Shelves ..............................................................................................................27
Control of Bacterial and Fungal Contaminants by Antibiotics in Plant Tissue Culture ............................................................................................................35
Procedure.................................................................................................................................37 Why Aeration is Essential ...............................................................................................38 Age of the Plant Tissue.......................................................................................................39 General Procedure ...............................................................................................................39 Suspension Cell Culture from Callus in vitro.............................................................42