Plant Tissue Culture: Totipotency to Transgenic

Plant Tissue Culture: Totipotency to Transgenic
20% Off

Plant Tissue Culture: Totipotency to Transgenic

295 Views
Publisher: Agrobios Publications
ISBN: 9788177544671
Author:
Availability: In Stock
INR 2,995.00 INR 2,396.00 ( 20% Off )
Effective Price after using Coupon Code: SAVE20
Download & Read Books Offline (Desktop/Laptop/Android Device) :
Customers who Bought this Ebook also Bought
  • Snapshot
  • Description

About this eBook

CONTENT
1. Plant Tissue Culture An Overview ................................................ 3 H. P. Sharma Reference.............................................................................................................9
2. Tissue Culture Laboratory Organization ........................................ 15 V. K. Shahi And Shailesh Kumar Introduction....................................................................................................... 15 Methods And Techniques Of Plant Tissue Culture............................................... 15 Organization Of A Tissue Culture Laboratory ..................................................... 16 1. Washing Area ................................................................................................ 17 2. Media Preparation Area ................................................................................. 18 3. Transfer Area ................................................................................................. 19 4. Culture Room ................................................................................................ 20 i. Control of Temperature .................................................................................................. 20 ii. Control of light ............................................................................................................. 20 iii. Arrangement of the shelves............................................................................................ 21 Equipments Used In Tissue Culture Laboratory .................................................. 22 Preparation Of Plant Tissue Culture Medium ...................................................... 24 Hardening Unit Greenhouse Facility.................................................................. 24 Observation And Data Collection Area ............................................................... 25 Packaging Area For Shipment ............................................................................ 26 Adoption Of Low-Cost Options For Plant Tissue Culture .................................... 26 Bioreactors as a low cost option for tissue culture.................................................................. 27 Aseptic Conditions In The Laboratory ................................................................ 27 i. Asepsis in the Facilities .................................................................................................. 27 ii. Asepsis in the Washing and Media Preparation Room ..................................................... 27 iii. Asepsis in the Transfer Room........................................................................................ 27 iv. Asepsis in the Culture Room ......................................................................................... 28 Personal Safety .................................................................................................. 28 References ......................................................................................................... 28
3. Sterilization Techniques in Plant Tissue Culture .............................. 31 Amarendra Narayan Misra1 And Meena Misra 2 Introduction....................................................................................................... 31 Aseptic Sterilization Technique........................................................................... 32 Sterilizing Culture Rooms And Transfer Hoods ................................................... 32 Sterilization Of Instruments And Equipments...................................................... 33 i. Steam Sterilization ........................................................................................................ 33 ii. Dry Sterilization........................................................................................................... 34 Post-Sterilisation Care Of The Instruments .......................................................... 35 Cleaning And Sterilising Glassware .................................................................... 35 Weighing Instruments ........................................................................................ 35 Liquid And Liquid Handling Systems ................................................................. 36 Sterilization Of Nutrient Media .......................................................................... 36 i. Steam Sterilization ........................................................................................................ 36 ii. Filter Sterilization ........................................................................................................ 37 Sterilization Of Plant Material ............................................................................ 38 Contaminants .................................................................................................................. 38 Conclusion ........................................................................................................ 41