Instrumental Methods of Analysis in Biotechnology

Instrumental Methods of Analysis in Biotechnology
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Instrumental Methods of Analysis in Biotechnology

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Publisher: IK International
ISBN: 9789381141380
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PREFACE Instrumental methods of analysis has importance in biotechnology and related fields, e.g., pharmaceutical science and biological science by applying various techniques and instruments in biological analysis and assay with the advancement of industrial applications and technology. This book is specifically written for the graduate and postgraduate students in biotechnology and other related biological sciences. The primary aim of this book is to provide a broad overview of methods and techniques used for the qualitative and quantitative analysis and assay of the respective biomolecules to give clear insight into the subject. This book has been written keeping in view the importance of various techniques and instruments used in biological analysis assay and industrial applications in biotechnology. This book is divided into eleven chapters. This book covers the various types of microscopes including electron microscopes, centrifugation techniques used in separation of nucleic acids, proteins and immunological substances, chromatographic techniques used in the separation of biological molecules with special reference to HPLC and GC techniques. The other part of this book covers the techniques used for isolation and purification of nucleic acids, their amplification using various PCR techniques, separation of nucleic acids, proteins and immunogenic substances with various electrophoretic techniques. Next chapters in this book focus on various spectrophotometric techniques used in biochemical analysis and mass spectroscopy covers the principles and applications of Raman, infra-red spectroscopy, NMR, ESR and magnetic resonance imaging MRI . The last part of this book includes radioisotopic techniques such as GM and scintillation counters and RIA, ELISA. This book also discusses the various approaches used in the determination of microbial growth with emphasis on FACS and Coulter counter techniques. This book will serve as a valuable source of information for students, teachers and researchers pursuing their study in biotechnology and other biological sciences. This is specially designed to cover the B.Sc. Hons Biotechnology and M.Sc. Biotechnology courses of various universities.
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CONTENTS PREFACE
We express our gratitude to Prof. T.C. Bhalla, Coordinator, Biotechnology Bioinformatics, H.P. University, Shimla for his benevolent guidance and encouragement to write this book. We express our thanks to Dr. Duni Chand, Chairman, Department of Biotechnology, Prof. S.S. Kanwar, Dr. Wamik Azmi, Dr. Reena Gupta, Dr. Arvind Kumar Bhatt, Principal Scientific Officer, Department of Biotechnology, H.P. State Govt. for his wishes. Thanks are due to the faculty members of AILS, Mandi, namely, Divya Thakur, Shipra Sharma, Neha Tandon, Sakshi Sharma, Aakriti Guleria, Shailja Guleria, Dr. Aditya Kumar, Dr. Suresh Kumar, Dr. Altaf Khan, Pankaj Sharma, Rojila Puri, Vandana, Dr. Yogesh Walia and many others for their wishes and valuable suggestions. Thanks are due to Prof. P.K. Khosla, Honb le Vice Chancellor, Shoolini